Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: A case against extrahepatic replication

Tomasz Laskus, Marek Radkowski, Lian Fu Wang, Janusz Cianciara, Hugo E Vargas, Jorge Rakela

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells (PBMCs) from 27 hepatitis C virus (HCV)-infected patients were analysed for the presence of HCV negative strand RNA with strand-specific Tth-based RT-PCR. No negative strand RNA was detected in any sample, and positive strand HCV sequences amplified from PBMCs were identical to those found in serum. These findings suggest that HCV does not replicate in PBMCs, and the presence of HCV sequences at this site is compatible with passive virus adsorption and/or contamination by circulating virus.

Original languageEnglish (US)
Pages (from-to)2747-2750
Number of pages4
JournalJournal of General Virology
Volume78
Issue number11
StatePublished - Nov 1997
Externally publishedYes

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Hepacivirus
Blood Cells
RNA
Serum
Viruses
Adsorption
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum : A case against extrahepatic replication. / Laskus, Tomasz; Radkowski, Marek; Wang, Lian Fu; Cianciara, Janusz; Vargas, Hugo E; Rakela, Jorge.

In: Journal of General Virology, Vol. 78, No. 11, 11.1997, p. 2747-2750.

Research output: Contribution to journalArticle

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