Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: A case against extrahepatic replication

Tomasz Laskus; Marek Radkowski; Lian Fu Wang; Janusz Cianciara; Hugo Vargas; Jorge Rakela

doi:10.1099/0022-1317-78-11-2747

Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: A case against extrahepatic replication

Tomasz Laskus, Marek Radkowski, Lian Fu Wang, Janusz Cianciara, Hugo Vargas, Jorge Rakela

Research output: Contribution to journal › Article › peer-review

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Abstract

Peripheral blood mononuclear cells (PBMCs) from 27 hepatitis C virus (HCV)-infected patients were analysed for the presence of HCV negative strand RNA with strand-specific Tth-based RT-PCR. No negative strand RNA was detected in any sample, and positive strand HCV sequences amplified from PBMCs were identical to those found in serum. These findings suggest that HCV does not replicate in PBMCs, and the presence of HCV sequences at this site is compatible with passive virus adsorption and/or contamination by circulating virus.

Original language	English (US)
Pages (from-to)	2747-2750
Number of pages	4
Journal	Journal of General Virology
Volume	78
Issue number	11
DOIs	https://doi.org/10.1099/0022-1317-78-11-2747
State	Published - Nov 1997

ASJC Scopus subject areas

Virology

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Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: A case against extrahepatic replication. / Laskus, Tomasz; Radkowski, Marek; Wang, Lian Fu et al.
In: Journal of General Virology, Vol. 78, No. 11, 11.1997, p. 2747-2750.

Research output: Contribution to journal › Article › peer-review

Laskus, T, Radkowski, M, Wang, LF, Cianciara, J, Vargas, H & Rakela, J 1997, 'Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: A case against extrahepatic replication', Journal of General Virology, vol. 78, no. 11, pp. 2747-2750. https://doi.org/10.1099/0022-1317-78-11-2747

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Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: A case against extrahepatic replication

Abstract

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