Hepatitis C virus in body fluids after liver transplantation

S. H. Caldwell, M. Sue, J. H. Bowden, Rolland Dickson, C. J. Driscoll, P. Yeaton, W. C. Stevenson, M. B. Ishitani, C. S. McCullough, T. L. Pruett, M. A. Lovell

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Recurrence of hepatitis C virus (HCV) after liver transplantation is common and is associated with high blood levels of HCV RNA. Higher blood levels of HCV may promote body fluid expression of the virus. We tested 152 body fluid specimens from 33 patients with chronic hepatitis C, 21 of whom had undergone prior liver transplantation. All patients had hepatitis C viremia, as determined by a reverse-transcription polymerase chain reaction (PCR) to the 5' noncoding region. The virus was quantitated in serum by the branched chain DNA assay (bDNA). Body fluids (33 sputum, 33 saliva, 33 urine, 32 tear, 9 vaginal, and 12 semen samples) were analyzed using PCR for HCV RNA. Serum HCV RNA by bDNA in the posttransplantation group was 255 ± 229 x 105 compared with 50 ± 56 x 105 eq/mL in the patients who did not undergo transplantation (P = .01). All urine, tear, and semen specimens were negative for HCV RNA. Five of 21 (24%) posttransplantation patients had detectable HCV RNA using PCR in oral secretions compared with 0 of 12 patients who did not undergo transplantation (not statistically significant). However, 5 of 11 patients with serum HCV RNA by bDNA results greater than 150 x 105 eq/mL had positive RNA in oral secretions compared to 0 of 22 patients with bDNA less than 150 x 105 eq/mL (P = .01). Posttransplantation patients were more likely to have bDNA levels exceeding 150 x 105 eq/mL (11 of 21 v 1 of 12, P = .03). Patients within the first year of transplantation were particularly prone to viral RNA levels exceeding 150 x 105 eq/mL (8 of 9 v 3 of 12, P = .01). We conclude that HCV RNA can occasionally be detected using PCR in oral secretions after liver transplantation. This is more likely during the first year posttransplantation when blood levels of HCV RNA often exceed 150 x 105 eq/mL by the branched chain DNA assay. Whether or not these observations represent an increased risk of transmission of infection during the early posttransplantation period is not certain.

Original languageEnglish (US)
Pages (from-to)124-129
Number of pages6
JournalLiver Transplantation and Surgery
Volume2
Issue number2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Body Fluids
Hepacivirus
Liver Transplantation
Branched DNA Signal Amplification Assay
RNA
Polymerase Chain Reaction
Transplantation
Semen
Tears
Serum
Urine
Viruses
Infectious Disease Transmission
Viremia
Viral RNA
Chronic Hepatitis C
DNA-Directed RNA Polymerases
Hepatitis C
Sputum
Saliva

ASJC Scopus subject areas

  • Surgery
  • Hepatology

Cite this

Caldwell, S. H., Sue, M., Bowden, J. H., Dickson, R., Driscoll, C. J., Yeaton, P., ... Lovell, M. A. (1996). Hepatitis C virus in body fluids after liver transplantation. Liver Transplantation and Surgery, 2(2), 124-129. https://doi.org/10.1002/lt.500020207

Hepatitis C virus in body fluids after liver transplantation. / Caldwell, S. H.; Sue, M.; Bowden, J. H.; Dickson, Rolland; Driscoll, C. J.; Yeaton, P.; Stevenson, W. C.; Ishitani, M. B.; McCullough, C. S.; Pruett, T. L.; Lovell, M. A.

In: Liver Transplantation and Surgery, Vol. 2, No. 2, 01.01.1996, p. 124-129.

Research output: Contribution to journalArticle

Caldwell, SH, Sue, M, Bowden, JH, Dickson, R, Driscoll, CJ, Yeaton, P, Stevenson, WC, Ishitani, MB, McCullough, CS, Pruett, TL & Lovell, MA 1996, 'Hepatitis C virus in body fluids after liver transplantation', Liver Transplantation and Surgery, vol. 2, no. 2, pp. 124-129. https://doi.org/10.1002/lt.500020207
Caldwell, S. H. ; Sue, M. ; Bowden, J. H. ; Dickson, Rolland ; Driscoll, C. J. ; Yeaton, P. ; Stevenson, W. C. ; Ishitani, M. B. ; McCullough, C. S. ; Pruett, T. L. ; Lovell, M. A. / Hepatitis C virus in body fluids after liver transplantation. In: Liver Transplantation and Surgery. 1996 ; Vol. 2, No. 2. pp. 124-129.
@article{c51cd2615cec4e6ca37d915542acd880,
title = "Hepatitis C virus in body fluids after liver transplantation",
abstract = "Recurrence of hepatitis C virus (HCV) after liver transplantation is common and is associated with high blood levels of HCV RNA. Higher blood levels of HCV may promote body fluid expression of the virus. We tested 152 body fluid specimens from 33 patients with chronic hepatitis C, 21 of whom had undergone prior liver transplantation. All patients had hepatitis C viremia, as determined by a reverse-transcription polymerase chain reaction (PCR) to the 5' noncoding region. The virus was quantitated in serum by the branched chain DNA assay (bDNA). Body fluids (33 sputum, 33 saliva, 33 urine, 32 tear, 9 vaginal, and 12 semen samples) were analyzed using PCR for HCV RNA. Serum HCV RNA by bDNA in the posttransplantation group was 255 ± 229 x 105 compared with 50 ± 56 x 105 eq/mL in the patients who did not undergo transplantation (P = .01). All urine, tear, and semen specimens were negative for HCV RNA. Five of 21 (24{\%}) posttransplantation patients had detectable HCV RNA using PCR in oral secretions compared with 0 of 12 patients who did not undergo transplantation (not statistically significant). However, 5 of 11 patients with serum HCV RNA by bDNA results greater than 150 x 105 eq/mL had positive RNA in oral secretions compared to 0 of 22 patients with bDNA less than 150 x 105 eq/mL (P = .01). Posttransplantation patients were more likely to have bDNA levels exceeding 150 x 105 eq/mL (11 of 21 v 1 of 12, P = .03). Patients within the first year of transplantation were particularly prone to viral RNA levels exceeding 150 x 105 eq/mL (8 of 9 v 3 of 12, P = .01). We conclude that HCV RNA can occasionally be detected using PCR in oral secretions after liver transplantation. This is more likely during the first year posttransplantation when blood levels of HCV RNA often exceed 150 x 105 eq/mL by the branched chain DNA assay. Whether or not these observations represent an increased risk of transmission of infection during the early posttransplantation period is not certain.",
author = "Caldwell, {S. H.} and M. Sue and Bowden, {J. H.} and Rolland Dickson and Driscoll, {C. J.} and P. Yeaton and Stevenson, {W. C.} and Ishitani, {M. B.} and McCullough, {C. S.} and Pruett, {T. L.} and Lovell, {M. A.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1002/lt.500020207",
language = "English (US)",
volume = "2",
pages = "124--129",
journal = "Liver Transplantation",
issn = "1527-6465",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Hepatitis C virus in body fluids after liver transplantation

AU - Caldwell, S. H.

AU - Sue, M.

AU - Bowden, J. H.

AU - Dickson, Rolland

AU - Driscoll, C. J.

AU - Yeaton, P.

AU - Stevenson, W. C.

AU - Ishitani, M. B.

AU - McCullough, C. S.

AU - Pruett, T. L.

AU - Lovell, M. A.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Recurrence of hepatitis C virus (HCV) after liver transplantation is common and is associated with high blood levels of HCV RNA. Higher blood levels of HCV may promote body fluid expression of the virus. We tested 152 body fluid specimens from 33 patients with chronic hepatitis C, 21 of whom had undergone prior liver transplantation. All patients had hepatitis C viremia, as determined by a reverse-transcription polymerase chain reaction (PCR) to the 5' noncoding region. The virus was quantitated in serum by the branched chain DNA assay (bDNA). Body fluids (33 sputum, 33 saliva, 33 urine, 32 tear, 9 vaginal, and 12 semen samples) were analyzed using PCR for HCV RNA. Serum HCV RNA by bDNA in the posttransplantation group was 255 ± 229 x 105 compared with 50 ± 56 x 105 eq/mL in the patients who did not undergo transplantation (P = .01). All urine, tear, and semen specimens were negative for HCV RNA. Five of 21 (24%) posttransplantation patients had detectable HCV RNA using PCR in oral secretions compared with 0 of 12 patients who did not undergo transplantation (not statistically significant). However, 5 of 11 patients with serum HCV RNA by bDNA results greater than 150 x 105 eq/mL had positive RNA in oral secretions compared to 0 of 22 patients with bDNA less than 150 x 105 eq/mL (P = .01). Posttransplantation patients were more likely to have bDNA levels exceeding 150 x 105 eq/mL (11 of 21 v 1 of 12, P = .03). Patients within the first year of transplantation were particularly prone to viral RNA levels exceeding 150 x 105 eq/mL (8 of 9 v 3 of 12, P = .01). We conclude that HCV RNA can occasionally be detected using PCR in oral secretions after liver transplantation. This is more likely during the first year posttransplantation when blood levels of HCV RNA often exceed 150 x 105 eq/mL by the branched chain DNA assay. Whether or not these observations represent an increased risk of transmission of infection during the early posttransplantation period is not certain.

AB - Recurrence of hepatitis C virus (HCV) after liver transplantation is common and is associated with high blood levels of HCV RNA. Higher blood levels of HCV may promote body fluid expression of the virus. We tested 152 body fluid specimens from 33 patients with chronic hepatitis C, 21 of whom had undergone prior liver transplantation. All patients had hepatitis C viremia, as determined by a reverse-transcription polymerase chain reaction (PCR) to the 5' noncoding region. The virus was quantitated in serum by the branched chain DNA assay (bDNA). Body fluids (33 sputum, 33 saliva, 33 urine, 32 tear, 9 vaginal, and 12 semen samples) were analyzed using PCR for HCV RNA. Serum HCV RNA by bDNA in the posttransplantation group was 255 ± 229 x 105 compared with 50 ± 56 x 105 eq/mL in the patients who did not undergo transplantation (P = .01). All urine, tear, and semen specimens were negative for HCV RNA. Five of 21 (24%) posttransplantation patients had detectable HCV RNA using PCR in oral secretions compared with 0 of 12 patients who did not undergo transplantation (not statistically significant). However, 5 of 11 patients with serum HCV RNA by bDNA results greater than 150 x 105 eq/mL had positive RNA in oral secretions compared to 0 of 22 patients with bDNA less than 150 x 105 eq/mL (P = .01). Posttransplantation patients were more likely to have bDNA levels exceeding 150 x 105 eq/mL (11 of 21 v 1 of 12, P = .03). Patients within the first year of transplantation were particularly prone to viral RNA levels exceeding 150 x 105 eq/mL (8 of 9 v 3 of 12, P = .01). We conclude that HCV RNA can occasionally be detected using PCR in oral secretions after liver transplantation. This is more likely during the first year posttransplantation when blood levels of HCV RNA often exceed 150 x 105 eq/mL by the branched chain DNA assay. Whether or not these observations represent an increased risk of transmission of infection during the early posttransplantation period is not certain.

UR - http://www.scopus.com/inward/record.url?scp=0029873351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029873351&partnerID=8YFLogxK

U2 - 10.1002/lt.500020207

DO - 10.1002/lt.500020207

M3 - Article

VL - 2

SP - 124

EP - 129

JO - Liver Transplantation

JF - Liver Transplantation

SN - 1527-6465

IS - 2

ER -