TY - JOUR
T1 - Hepatic insulin sensitivity in healthy and prediabetic subjects
T2 - From a dual- to a single-tracer oral minimal model
AU - Visentin, Roberto
AU - Dalla Man, Chiara
AU - Basu, Rita
AU - Basu, Ananda
AU - Rizza, Robert A.
AU - Cobelli, Claudio
N1 - Publisher Copyright:
© 2015 the American Physiological Society.
PY - 2015/7/15
Y1 - 2015/7/15
N2 - Recently, a model was proposed to assess hepatic insulin sensitivity during a meal, i.e., the ability of insulin to suppress glucose production (EGP), SIP. The model was developed on EGP data obtained from a triple-tracer meal and the tracer-to-tracee clamp technique and validated against the euglycemic hyperinsulinemic clamp. The aim of this study was to assess whether SIP can be obtained from plasma concentrations measured after a single-tracer meal by incorporating the above EGP model into the oral glucose minimal model by describing both glucose production and disposal (OMMPD). Triple-tracer meal data of two databases (20 healthy and 60 healthy and prediabetic subjects) were used. Virtually model-independent EGP estimates were calculated. OMMPD was identified on exogenous and endogenous glucose concentrations, providing indices of SIP, disposal insulin sensitivity (SID), and EGP. The model fitted the data well, and SP and were estimated with precision in both databases (SIp = 5.48 ± 0.54 10-4 dl-kg-1-min-1 per μU/ml and SID = 9.93 ± 2.18 10-4 dl-kg-1-min-1per μU/ml in healthy; SIp = 5.41 ± 3.55 10-4 dl-kg-1-min-1 per μU/ml and SID = 5.34 ± 6.17 10-4 dl-kg-1-min-1 per μU/ml, in healthy and prediabetic subjects). Estimated SIp and that derived from the triple-tracer EGP model were very similar on average. Moreover, the time course of EGP normalized to basal EGP (EGPb), and EGP/EGPb agreed with the results obtained using the triple-tracer method. In this study, we have demonstrated that SIP,SID, and EGP/EGPb time course can be estimated reliably from a single-tracer meal protocol in both healthy and prediabetic subjects.
AB - Recently, a model was proposed to assess hepatic insulin sensitivity during a meal, i.e., the ability of insulin to suppress glucose production (EGP), SIP. The model was developed on EGP data obtained from a triple-tracer meal and the tracer-to-tracee clamp technique and validated against the euglycemic hyperinsulinemic clamp. The aim of this study was to assess whether SIP can be obtained from plasma concentrations measured after a single-tracer meal by incorporating the above EGP model into the oral glucose minimal model by describing both glucose production and disposal (OMMPD). Triple-tracer meal data of two databases (20 healthy and 60 healthy and prediabetic subjects) were used. Virtually model-independent EGP estimates were calculated. OMMPD was identified on exogenous and endogenous glucose concentrations, providing indices of SIP, disposal insulin sensitivity (SID), and EGP. The model fitted the data well, and SP and were estimated with precision in both databases (SIp = 5.48 ± 0.54 10-4 dl-kg-1-min-1 per μU/ml and SID = 9.93 ± 2.18 10-4 dl-kg-1-min-1per μU/ml in healthy; SIp = 5.41 ± 3.55 10-4 dl-kg-1-min-1 per μU/ml and SID = 5.34 ± 6.17 10-4 dl-kg-1-min-1 per μU/ml, in healthy and prediabetic subjects). Estimated SIp and that derived from the triple-tracer EGP model were very similar on average. Moreover, the time course of EGP normalized to basal EGP (EGPb), and EGP/EGPb agreed with the results obtained using the triple-tracer method. In this study, we have demonstrated that SIP,SID, and EGP/EGPb time course can be estimated reliably from a single-tracer meal protocol in both healthy and prediabetic subjects.
KW - Glucose kinetics
KW - Insulin resistance
KW - Meal
KW - Tracer
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U2 - 10.1152/ajpendo.00358.2014
DO - 10.1152/ajpendo.00358.2014
M3 - Article
C2 - 25991649
AN - SCOPUS:84937541365
SN - 0193-1849
VL - 309
SP - E161-E167
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 2
ER -