Hemopoietic histocompatibility (Hh-1) regulatory and structural genes of the f haplotype map to H-2

R. M. Rembecki, V. Kumar, C. S. David, M. Bennett

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Natural resistance to bone marrow stem cell (BMC) grafts in lethally irradiated mice is a consequence of natural killer cell recognition and elimination of BMC that express hemopoietic histocompatibility (Hh) antigens inherited noncodominantly. The major Hh genetic region, Hh-1, maps to H-2. The phenotype of the BMC was determined by grafting BMC into panels of irradiated mice, and measuring splenic incorporation of the radioactive specific DNA precursor, 5-iodo-2'-deoxyuridine [125I] (IUdR) 5 days after cell transfer. Our previous analysis of Hh-1 antigen expression on BMC of intra-H-2 recombinant inbred strain mice indicated that Hh-1 regulatory genes (Hh-1r) map in the H-2S/H-2D interval. Nine recombinants described here shared the following: (1) the DL region was donated by H-2' that was associated with expression of determinant 2, shared with H-2(d)/Hh-1(d), and (2) had crossovers in the S/D or E/S intervals or within E(b), and (3) had BMC that were Hh-1 null. F1 hybrids of crosses between mice of these recombinant strains with H-2(d) strain mice had BMC that expressed determinant 2, suggesting that a structural gene had been lost by the crossover event. Crosses with H-2b strain mice produced mice whose BMC were Hh-1 null, indicating that the H-2(f)/Hh-1(f) regulatory genes were still intact. We suggest that Hh-1r maps to the S/D interval of all haplotypes so far studied, even H-2(f). The structural gene for the f haplotype is centromeric of E(b) and may be K(f), based on recent data supporting the role of class I antigens in Hh antigen expression.

Original languageEnglish (US)
Pages (from-to)633-638
Number of pages6
JournalTransplantation
Volume49
Issue number3
StatePublished - 1990

Fingerprint

Histocompatibility
Regulator Genes
Bone Marrow Cells
Haplotypes
Stem Cells
Histocompatibility Antigens
Idoxuridine
Histocompatibility Antigens Class I
Inbred Strains Mice
Innate Immunity
Natural Killer Cells
Genes
Transplants
Phenotype
DNA

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Rembecki, R. M., Kumar, V., David, C. S., & Bennett, M. (1990). Hemopoietic histocompatibility (Hh-1) regulatory and structural genes of the f haplotype map to H-2. Transplantation, 49(3), 633-638.

Hemopoietic histocompatibility (Hh-1) regulatory and structural genes of the f haplotype map to H-2. / Rembecki, R. M.; Kumar, V.; David, C. S.; Bennett, M.

In: Transplantation, Vol. 49, No. 3, 1990, p. 633-638.

Research output: Contribution to journalArticle

Rembecki, RM, Kumar, V, David, CS & Bennett, M 1990, 'Hemopoietic histocompatibility (Hh-1) regulatory and structural genes of the f haplotype map to H-2', Transplantation, vol. 49, no. 3, pp. 633-638.
Rembecki, R. M. ; Kumar, V. ; David, C. S. ; Bennett, M. / Hemopoietic histocompatibility (Hh-1) regulatory and structural genes of the f haplotype map to H-2. In: Transplantation. 1990 ; Vol. 49, No. 3. pp. 633-638.
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