Hemodynamic and renal effects of cross-linked hemoglobin infusion

Aleix Cases, John M. Stulak, Zvonimir S Katusic, Eduardo Villa, J. C. Romero

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

It is well known that hemoglobin binds nitric oxide (NO) and produces a pronounced vasoconstriction in isolated arteries. However, it is debatable whether such an effect takes place in whole animals, because hemoglobin also catalyzes the formation of prostaglandins from arachidonic acid. Short-term studies were performed to evaluate the effects induced by intravenous infusion of cross-linked hemoglobin (XL-Hb) on blood pressure (BP) and renal, iliac, and mesenteric flows, and on renal function in six anesthetized dogs. A similar volume-matched expansion with 6% dextran was used as a control (n = 6). Glomerular filtration rate (GFR), urinary flow, and total and fractional sodium excretion were measured before and after XL-Hb or dextran infusion to evaluate possible renal function changes. XL-Hb administration resulted in a 29% elevation in BP and a significant decrease of blood flow (30-37%) to the three vascular beds. XL-Hb did not alter GFR or sodium excretion, despite the increase in BP. In contrast, the administration of dextran did not significantly alter BP but induced a significant increase (6-13%) of blood flow in the three vascular beds. These changes were accompanied by threefold increases in urinary flow and sodium excretion without alterations in GFR. The binding effect of XL-Hb on NO was studied in isolated renal arteries in organ chambers. These in vitro studies showed that XL-Hb blunted the endothelium-mediated vasodilator response to calcium ionophore A-23187 and to acetylcholine. Our results demonstrate that XL-Hb administration is followed by hypertension, vasoconstriction, and blunted natriuresis. All these effects are compatible with the scavenging effect on NO attributed to XL-Hb.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume272
Issue number3 41-3
StatePublished - Mar 1997

Fingerprint

Hemoglobins
Dextrans
Hemodynamics
Glomerular Filtration Rate
Blood Pressure
Kidney
Sodium
Vasoconstriction
Blood Vessels
Nitric Oxide
Natriuresis
Calcium Ionophores
Calcimycin
Renal Artery
Vasodilator Agents
Intravenous Infusions
Arachidonic Acid
Acetylcholine
Prostaglandins
Endothelium

Keywords

  • dextran
  • nitric oxide
  • prostaglandins

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Hemodynamic and renal effects of cross-linked hemoglobin infusion. / Cases, Aleix; Stulak, John M.; Katusic, Zvonimir S; Villa, Eduardo; Romero, J. C.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 272, No. 3 41-3, 03.1997.

Research output: Contribution to journalArticle

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