Hemisection spinal cord injury in rat: The value of intraoperative somatosensory evoked potential monitoring

Beth A. Cloud, Bret G. Ball, Bingkun K. Chen, Andrew M. Knight, Jeffrey S. Hakim, Ana M. Ortiz, Anthony John Windebank

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Techniques used to produce partial spinal cord injuries in animal models have the potential for creating variability in lesions. The amount of tissue affected may influence the functional outcomes assessed in the animals. The recording of somatosensory evoked potentials (SSEPs) may be a valuable tool for assessing the extent of lesion applied in animal models of traumatic spinal cord injury (SCI). Intraoperative tibial SSEP recordings were assessed during surgically induced lateral thoracic hemisection SCI in Sprague-Dawley rats. The transmission of SSEPs, or lack thereof, was determined and compared against the integrity of the dorsal funiculi on each side of the spinal cord upon histological sectioning. An association was found between the presence of an SSEP signal and presence of intact dorsal funiculus tissue. The relative risk is 4.50 (95% confidence interval: 1.83-11.08) for having an intact dorsal funiculus when the ipsilateral SSEP was present compared to when it was absent. Additionally, the amount of spared spinal cord tissue correlates with final functional assessments at nine weeks post injury: BBB (linear regression, R2=0.618, p<0.001) and treadmill test (linear regression, R2=0.369, p=0.016). Therefore, we propose intraoperative SSEP monitoring as a valuable tool to assess extent of lesion and reduce variability between animals in experimental studies of SCI.

Original languageEnglish (US)
Pages (from-to)179-184
Number of pages6
JournalJournal of Neuroscience Methods
Volume211
Issue number2
DOIs
StatePublished - Nov 15 2012

Fingerprint

Somatosensory Evoked Potentials
Spinal Cord Injuries
Linear Models
Spinal Cord
Animal Models
Exercise Test
Sprague Dawley Rats
Thorax
Confidence Intervals
Wounds and Injuries

Keywords

  • Electrophysiology
  • Exercise
  • Functional outcome
  • Incomplete spinal cord injury
  • Pathologic correlation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hemisection spinal cord injury in rat : The value of intraoperative somatosensory evoked potential monitoring. / Cloud, Beth A.; Ball, Bret G.; Chen, Bingkun K.; Knight, Andrew M.; Hakim, Jeffrey S.; Ortiz, Ana M.; Windebank, Anthony John.

In: Journal of Neuroscience Methods, Vol. 211, No. 2, 15.11.2012, p. 179-184.

Research output: Contribution to journalArticle

Cloud, Beth A. ; Ball, Bret G. ; Chen, Bingkun K. ; Knight, Andrew M. ; Hakim, Jeffrey S. ; Ortiz, Ana M. ; Windebank, Anthony John. / Hemisection spinal cord injury in rat : The value of intraoperative somatosensory evoked potential monitoring. In: Journal of Neuroscience Methods. 2012 ; Vol. 211, No. 2. pp. 179-184.
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