Heme: A novel inducer of MCP-1 through HO-dependent and HO-independent mechanisms

S. K R Kanakiriya, Anthony J. Croatt, Jill J. Haggard, Julie R. Ingelfinger, Shiow Shih Tang, Jawed Alam, Karl A Nath

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Abstract

This study examined the effect of hemin on the expression of heme oxygenase-1 (HO-1) and monocyte chemoattractant protein-1 (MCP-1) in immortalized rat proximal tubular epithelial cells (IRPTCs). Hemin elicited a dose- and time-dependent induction of HO-1 and MCP-1 mRNA. HO activity contributed to MCP-1 mRNA expression at early time points (4-6 h) because inhibition of HO activity by zinc protoporphyrin (ZnPP) prevented hemin-induced expression of MCP-1 mRNA. Catalytically active intracellular iron was markedly increased in hemin-treated IRPTCs and contributed to the induction of HO-1 and MCP-1 mRNA because an iron chelator blocked hemin-induced upregulation of both genes, whereas a cell-permeant form of iron directly induced these genes. N-acetylcysteine completely blocked hemin-induced expression of HO-1 and MCP-1 mRNA, thereby providing added evidence for redox regulation of expression of these genes. The redox-sensitive transcription factor NF-κB was recruited in hemin-induced upregulation of MCP-1 because two different compounds that abrogate the activation of NF-κB (TPCK and BAY 11-7082) completely blocked hemin-induced upregulation of MCP-1 mRNA. In contrast to this HO-mediated induction of MCP-1 through redox-sensitive, iron-dependent, and NF-κB-involved pathways observed after 4-6 h, hemin also elicited a delayed induction of MCP-1 at 18 h through HO-independent pathways. We conclude that hemin is a potent inducer of MCP-1 in IRPTCs: HO-dependent, hemedegrading pathways lead to an early, robust, and selfremitting induction of MCP-1, whereas HO-independent mechanisms lead to a delayed expression of MCP-1.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume284
Issue number3 53-3
StatePublished - Mar 1 2003

Fingerprint

Chemokine CCL2
Heme
Hemin
Heme Oxygenase-1
Messenger RNA
Iron
Oxidation-Reduction
Up-Regulation
Epithelial Cells
Tosylphenylalanyl Chloromethyl Ketone
Acetylcysteine
Gene Expression Regulation
Chelating Agents
Genes
Transcription Factors

Keywords

  • Heme oxygenase
  • Iron
  • Monocyte chemoattractant protein-1
  • Oxidant stress

ASJC Scopus subject areas

  • Physiology

Cite this

Kanakiriya, S. K. R., Croatt, A. J., Haggard, J. J., Ingelfinger, J. R., Tang, S. S., Alam, J., & Nath, K. A. (2003). Heme: A novel inducer of MCP-1 through HO-dependent and HO-independent mechanisms. American Journal of Physiology - Renal Physiology, 284(3 53-3).

Heme : A novel inducer of MCP-1 through HO-dependent and HO-independent mechanisms. / Kanakiriya, S. K R; Croatt, Anthony J.; Haggard, Jill J.; Ingelfinger, Julie R.; Tang, Shiow Shih; Alam, Jawed; Nath, Karl A.

In: American Journal of Physiology - Renal Physiology, Vol. 284, No. 3 53-3, 01.03.2003.

Research output: Contribution to journalArticle

Kanakiriya, SKR, Croatt, AJ, Haggard, JJ, Ingelfinger, JR, Tang, SS, Alam, J & Nath, KA 2003, 'Heme: A novel inducer of MCP-1 through HO-dependent and HO-independent mechanisms', American Journal of Physiology - Renal Physiology, vol. 284, no. 3 53-3.
Kanakiriya SKR, Croatt AJ, Haggard JJ, Ingelfinger JR, Tang SS, Alam J et al. Heme: A novel inducer of MCP-1 through HO-dependent and HO-independent mechanisms. American Journal of Physiology - Renal Physiology. 2003 Mar 1;284(3 53-3).
Kanakiriya, S. K R ; Croatt, Anthony J. ; Haggard, Jill J. ; Ingelfinger, Julie R. ; Tang, Shiow Shih ; Alam, Jawed ; Nath, Karl A. / Heme : A novel inducer of MCP-1 through HO-dependent and HO-independent mechanisms. In: American Journal of Physiology - Renal Physiology. 2003 ; Vol. 284, No. 3 53-3.
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