Hematoporphyrin derivative photocytotoxicity of human glioblastoma in cell culture

R. E. Wharen, S. So, R. E. Anderson, E. R. Laws

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The parameters of hematoporphyrin-derivative (HpD) photocytotoxicity of human glioma cells in cell culture were studied to determine the optimum wavelength and power density of light, to investigate the influence of tissue oxygenation, and to evaluate the role of singlet oxygen and free radicals in producing cell death. Cell survival curves demonstrated a relative killing efficiency of 12:1 for violet compared to red light. Eighty joules of red light were required to produce 100% cell kill at an HpD concentration of 10 μg/ml, a level of HpD that has been quantitated in biopsies from patients receiving HpD photoradiation therapy. No difference in cellular killing efficiency was observed for power densities of red light varying from 10 to 100 mW/cm 2. Cytotoxicity was directly related to O 2 tension from 12 to 490 torr with a slight but consistent increase in cell kill at O 2 tensions from 7 to 12 torr. Cytotoxicity was effectively quenched by β-carotene, whereas mannitol had no effect, indicating that cytotoxicity is probably mediated via a mechanism involving singlet oxygen. This information may serve as a basis for more effective application of HpD photoradiation therapy and for designing protocols to study the efficacy of such therapy.

Original languageEnglish (US)
Pages (from-to)495-501
Number of pages7
JournalNeurosurgery
Volume19
Issue number4
StatePublished - 1986
Externally publishedYes

Fingerprint

Hematoporphyrin Derivative
Glioblastoma
Cell Culture Techniques
Hematoporphyrin Photoradiation
Light
Singlet Oxygen
Phototherapy
Mannitol
Carotenoids
Glioma
Free Radicals
Reactive Oxygen Species
Cell Survival
Cell Death
Biopsy

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Wharen, R. E., So, S., Anderson, R. E., & Laws, E. R. (1986). Hematoporphyrin derivative photocytotoxicity of human glioblastoma in cell culture. Neurosurgery, 19(4), 495-501.

Hematoporphyrin derivative photocytotoxicity of human glioblastoma in cell culture. / Wharen, R. E.; So, S.; Anderson, R. E.; Laws, E. R.

In: Neurosurgery, Vol. 19, No. 4, 1986, p. 495-501.

Research output: Contribution to journalArticle

Wharen, RE, So, S, Anderson, RE & Laws, ER 1986, 'Hematoporphyrin derivative photocytotoxicity of human glioblastoma in cell culture', Neurosurgery, vol. 19, no. 4, pp. 495-501.
Wharen, R. E. ; So, S. ; Anderson, R. E. ; Laws, E. R. / Hematoporphyrin derivative photocytotoxicity of human glioblastoma in cell culture. In: Neurosurgery. 1986 ; Vol. 19, No. 4. pp. 495-501.
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