Hematologic effects of stem cell factor in vivo and in vitro in rodents

T. R. Ulich, J. Del Castillo, E. S. Yi, S. Yin, I. McNiece, Y. P. Yung, Y. P. Zsebo

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Recombinant rat stem cell factor (rrSCF) administered to rats as a single intravenous injection causes a dose-dependent neutrophilia and lymphocytosis as well as the appearance of immature myeloid cells and occasional blast cells in the circulation. Neutrophilia begins at 2 hours, peaks at 4 to 6 hours, and subsides between 12 and 24 hours. Lymphocytosis occurs at 0.5 hours and has subsided by 2 hours. rrSCF-induced neutrophilia and lymphocytosis are abrogated by boiling, demonstrating that endotoxin-contamination of the rrSCF preparation is not responsible for the observed hematologic effects. The bone marrow at 6 hours after injection of rrSCF shows a left-shifted myeloid and erythroid hyperplasia as evidenced by significant increases in the absolute numbers of morphologically recognizable early myeloid and erythroid precursors. A concurrent decrease in the absolute numbers of mature marrow neutrophils is noted, suggesting that the release of marrow neutrophils contributes to the peripheral neutrophilia. After 2 weeks of daily injections of rrSCF, bone marrow smears demonstrate a remarkable mast cell hyperplasia accompanied by a decrease in total marrow cellularity and by a striking erythroid and lymphoid hypoplasia. rrSCF also causes mast cells to appear in the circulation and causes a systemic increase in embryonic connective tissue-type, but not mucosal-type, mast cells. In vitro long-term culture of lineage-depleted mouse bone marrow cells with rrSCF results in an almost pure outgrowth of mast cells.

Original languageEnglish (US)
Pages (from-to)645-650
Number of pages6
JournalBlood
Volume78
Issue number3
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Ulich, T. R., Del Castillo, J., Yi, E. S., Yin, S., McNiece, I., Yung, Y. P., & Zsebo, Y. P. (1991). Hematologic effects of stem cell factor in vivo and in vitro in rodents. Blood, 78(3), 645-650. https://doi.org/10.1182/blood.v78.3.645.bloodjournal783645