Hemagglutinin protein is a primary target of the measles virus-specific HLA-A2-restricted CD8+ T cell response during measles and after vaccination

Martin O. Ota, Zaza Ndhlovu, SangKon Oh, Sucheep Piyasirisilp, Jay A. Berzofsky, William J. Moss, Diane E. Griffin

Research output: Contribution to journalArticle

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Abstract

To characterize the measles virus (MV)-specific T cell responses important for evaluation of measles vaccines, human leukocyte antigen (HLA)-A2-positive and -negative adults immunized with measles-mumps-rubella vaccine were studied. Both groups developed increases in antibody and in interferon (IFN)-γ-producing cells in response to pooled hemagglutinin (H) and fusion peptides. HLA-A2-binding peptides were predicted for all MV-encoded proteins and confirmed by T2 cell stabilization. Twenty-nine peptides were tested, and 19 (6 from H) stimulated increased IFN-γ secretion in a majority of vaccinees. Peptide-loaded HLA-A2 tetramers or immunoglobulin dimers documented MV-specific CD8+ T cell responses after vaccination and during measles and confirmed new A2 epitopes in H (250-259 and 516-525 aa) and matrix (M; 50-58 aa) protein and previously described epitopes in H (30-38 aa), M (211-219 aa), and nonstructural protein C (84-92 aa). No single peptide dominated the response. We conclude that H is an important stimulus for CD8 + T cell as well as for antibody responses in HLA-A2-positive individuals.

Original languageEnglish (US)
Pages (from-to)1799-1807
Number of pages9
JournalJournal of Infectious Diseases
Volume195
Issue number12
DOIs
StatePublished - Jun 15 2007
Externally publishedYes

Fingerprint

Measles virus
Measles
Hemagglutinins
HLA Antigens
varespladib methyl
Vaccination
T-Lymphocytes
Peptides
Interferons
Epitopes
Proteins
Measles-Mumps-Rubella Vaccine
Measles Vaccine
Protein C
Antibody Formation
Immunoglobulins
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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Hemagglutinin protein is a primary target of the measles virus-specific HLA-A2-restricted CD8+ T cell response during measles and after vaccination. / Ota, Martin O.; Ndhlovu, Zaza; Oh, SangKon; Piyasirisilp, Sucheep; Berzofsky, Jay A.; Moss, William J.; Griffin, Diane E.

In: Journal of Infectious Diseases, Vol. 195, No. 12, 15.06.2007, p. 1799-1807.

Research output: Contribution to journalArticle

Ota, Martin O. ; Ndhlovu, Zaza ; Oh, SangKon ; Piyasirisilp, Sucheep ; Berzofsky, Jay A. ; Moss, William J. ; Griffin, Diane E. / Hemagglutinin protein is a primary target of the measles virus-specific HLA-A2-restricted CD8+ T cell response during measles and after vaccination. In: Journal of Infectious Diseases. 2007 ; Vol. 195, No. 12. pp. 1799-1807.
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