Heligmosomoides polygyrus adult worm homogenate superantigen: Presentation to T cells requires MHC class I positive accessory cells

Michael Robinson, Thomas R. Gustad, Neil Storey, Chella S. David

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A series of experiments were carried out to further characterize the previously discovered Heligmosomoides polygyrus, adult worm homogenate (AWH), superantigen. AWH, in contrast to staphylococcal enterotoxin B (SEB) superantigen, was totally unable to stimulate naive thymocytes, in either the presence or the absence of exogenous accessory cells (AC). Experiments using AC from B10 congenic mice failed to indicate a requirement for a specific MHC haplotype for successful presentation of the AWH superantigen and also indicated that the presence or absence of the H-2,E molecule on AC did not affect AWH stimulation of T cell hybridomas. Furthermore, AWH was found to require the presence of MHC Class I-positive AC in order to stimulate T cell hybridomas, while, in contrast, the absence of MHC Class II on AC did not affect the superantigenic properties of AWH. Initial characterization of the T cell hybridomas stimulated by the AWH superantigen, indicated that all were CD4-positive and that three of them expressed TCR V(β)8.1. Hence AWH superantigen can stimulate TCR V(β)8.1/CD4-positive T cells only in the presence of MHC Class I-positive AC.

Original languageEnglish (US)
Pages (from-to)188-194
Number of pages7
JournalCellular Immunology
Volume161
Issue number2
DOIs
StatePublished - 1995

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Nematospiroides dubius
Superantigens
T-Lymphocytes
Hybridomas
Congenic Mice
Thymocytes
Haplotypes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Heligmosomoides polygyrus adult worm homogenate superantigen : Presentation to T cells requires MHC class I positive accessory cells. / Robinson, Michael; Gustad, Thomas R.; Storey, Neil; David, Chella S.

In: Cellular Immunology, Vol. 161, No. 2, 1995, p. 188-194.

Research output: Contribution to journalArticle

Robinson, Michael ; Gustad, Thomas R. ; Storey, Neil ; David, Chella S. / Heligmosomoides polygyrus adult worm homogenate superantigen : Presentation to T cells requires MHC class I positive accessory cells. In: Cellular Immunology. 1995 ; Vol. 161, No. 2. pp. 188-194.
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