TY - JOUR
T1 - Heligmosomoides polygyrus adult worm homogenate superantigen
T2 - Presentation to T cells requires MHC class I positive accessory cells
AU - Robinson, Michael
AU - Gustad, Thomas R.
AU - Storey, Nell
AU - David, Chella S.
PY - 1995/4/1
Y1 - 1995/4/1
N2 - A series of experiments were carried out to further characterize the previously discovered Heligmosomoides polygyrus, adult worm homogenate (AWH), superantigen. AWH, in contrast to staphylococcal enterotoxin B (SEB) superantigen, was totally unable to stimulate naive thymocytes, in either the presence or the absence of exogenous accessory cells (AC). Experiments using AC from B10 congenic mice failed to indicate a requirement for a specific MHC haplotype for successful presentation of the AWH superantigen and also indicated that the presence or absence of the H-2, E molecule on AC did not affect AWH stimulation of T cell hybridamos. Furthermore, AWH was found to require the presence of MHC Class I-positive AC in order to stimulate T cell hybridomas, while, in contrast, the absence of MHC Class II on AC did not affect the superantigenic properties of AWH. Initial characterization of the T cell hybridomas stimulated by the AWH superantigen, indicated that all were CD4-positive and that three of them expressed TCR Vβ8.1. Hence AWH superantigen can stimulate TCR Vβ8.1/CD4-positive T cells only in the presence of MHC Class I-positive AC.
AB - A series of experiments were carried out to further characterize the previously discovered Heligmosomoides polygyrus, adult worm homogenate (AWH), superantigen. AWH, in contrast to staphylococcal enterotoxin B (SEB) superantigen, was totally unable to stimulate naive thymocytes, in either the presence or the absence of exogenous accessory cells (AC). Experiments using AC from B10 congenic mice failed to indicate a requirement for a specific MHC haplotype for successful presentation of the AWH superantigen and also indicated that the presence or absence of the H-2, E molecule on AC did not affect AWH stimulation of T cell hybridamos. Furthermore, AWH was found to require the presence of MHC Class I-positive AC in order to stimulate T cell hybridomas, while, in contrast, the absence of MHC Class II on AC did not affect the superantigenic properties of AWH. Initial characterization of the T cell hybridomas stimulated by the AWH superantigen, indicated that all were CD4-positive and that three of them expressed TCR Vβ8.1. Hence AWH superantigen can stimulate TCR Vβ8.1/CD4-positive T cells only in the presence of MHC Class I-positive AC.
UR - http://www.scopus.com/inward/record.url?scp=0028963628&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028963628&partnerID=8YFLogxK
U2 - 10.1006/cimm.1995.1026
DO - 10.1006/cimm.1995.1026
M3 - Article
C2 - 7697729
AN - SCOPUS:0028963628
SN - 0008-8749
VL - 161
SP - 188
EP - 194
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -