TY - JOUR
T1 - Heated hand vein blood is satisfactory for measurements during free fatty acid kinetic studies
AU - Jensen, Michael D.
AU - Heiling, Valarie J.
N1 - Funding Information:
From the Endocrine Research Unit, Depattment of Medicine, Mayo Clinic, Rochester, MN. Supported by Grants No. DK40484, DK38092, and RR-0585 from the US Public Health Service and by the Mayo Foundation. Address reprint requests to Michael D. Jensen, MD, Endocrine Research Unit, 5-164 West Joseph, Rochester, MN 55905. Copyright 0 1991 by W.B . Saunders Company 00260495l91l4004-0012$03.00/0
PY - 1991/4
Y1 - 1991/4
N2 - Quantitatively accurate measurements of plasma free fatty acid (FFA) turnover can be made if the FFA tracer is infused intravenously and arterial blood is sampled. To be certain that arterialized-venous blood provides comparable information, plasma FFA concentration and specific activity were measured in both arterial and arterialized-venous blood in 19 volunteers undergoing infusion of [1-14C]palmitate infusion before and during a euglycemic, hyperinsulinemic clamp. Heated hand vein blood was obtained using either the heated box (n = 9 subjects) or heating pad (n = 10 subjects) technique. The heated box arterialized-venous blood had palmitate concentration and specific activity equal to arterial blood. The use of the heating pad resulted in small (3%), but statistically significant (P < .05), underestimates of palmitate specific activity. In addition, plasma lactate concentrations were significantly higher in heated hand vein blood than arterial blood. In summary, heated hand vein blood is a satisfactory alternative to arterial blood for tracer studies of FFA metabolism.
AB - Quantitatively accurate measurements of plasma free fatty acid (FFA) turnover can be made if the FFA tracer is infused intravenously and arterial blood is sampled. To be certain that arterialized-venous blood provides comparable information, plasma FFA concentration and specific activity were measured in both arterial and arterialized-venous blood in 19 volunteers undergoing infusion of [1-14C]palmitate infusion before and during a euglycemic, hyperinsulinemic clamp. Heated hand vein blood was obtained using either the heated box (n = 9 subjects) or heating pad (n = 10 subjects) technique. The heated box arterialized-venous blood had palmitate concentration and specific activity equal to arterial blood. The use of the heating pad resulted in small (3%), but statistically significant (P < .05), underestimates of palmitate specific activity. In addition, plasma lactate concentrations were significantly higher in heated hand vein blood than arterial blood. In summary, heated hand vein blood is a satisfactory alternative to arterial blood for tracer studies of FFA metabolism.
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U2 - 10.1016/0026-0495(91)90152-M
DO - 10.1016/0026-0495(91)90152-M
M3 - Article
C2 - 2011082
AN - SCOPUS:0025727792
SN - 0026-0495
VL - 40
SP - 406
EP - 409
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 4
ER -