Heat shock gene-expression in HSP-70 and HSF1 gene-transfected human epidermoid A-431 cells

X. Z. Ding, G. C. Tsokos, Robert Christian Smallridge, J. G. Kiang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Thermotolerant cells display attenuated heat shock protein 70 kD (HSP-70) gene expression and signal transduction such as intracellular Ca2+ concentration and inositol trisphosphate in response to sublethal heat. To further investigate the regulation of heat shock gene expression, we developed constructs containing human HSP-70 and HSF1 genes and transfected human epidermoid A-431 cells. These cells were chosen because skin cells are especially vulnerable to heat shock and other environmental stressors. We report that A431 cells can be successfully transfected with HSP-70 and HSF1 genes as shown by the elevated levels of respective message and protein. Overexpression of HSP-70 in cells transfected with HSP-70 gene led to a down-regulation of the HSF1 gene expression. Interestingly, transfection of cells with the HSF1 gene was not associated with increased expression of HSP-70. Exposure of HSF1 gene-transfected cells to heat resulted in a transient but significant increase in HSP-70 gene expression as compared to that found in vector-transfected cells, which was completely inhibited by treatment with staurosporine. In conclusion, we have demonstrated successful transfection of human A-431 cells with HSF1 and HSP-70 genes, where the regulation of their expression can be studied.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalMolecular and Cellular Biochemistry
Volume167
Issue number1-2
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

HSP70 Heat-Shock Proteins
Gene expression
Shock
Hot Temperature
Genes
Gene Expression
Gene Expression Regulation
Transfection
Signal transduction
Staurosporine
Inositol
Skin
Signal Transduction
Down-Regulation

Keywords

  • Epithelia
  • Heat shock factor 1
  • Heat shock protein 70 kd
  • mRNA
  • RT-PCR
  • Transfection

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Heat shock gene-expression in HSP-70 and HSF1 gene-transfected human epidermoid A-431 cells. / Ding, X. Z.; Tsokos, G. C.; Smallridge, Robert Christian; Kiang, J. G.

In: Molecular and Cellular Biochemistry, Vol. 167, No. 1-2, 1997, p. 145-152.

Research output: Contribution to journalArticle

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