Head-to-tail primer tandem repeats generated in hemi-nested PCR

L. F. Wang; J. Rakela; T. Laskus

doi:10.1006/mcpr.1997.0122

Head-to-tail primer tandem repeats generated in hemi-nested PCR

L. F. Wang, J. Rakela, T. Laskus

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Evidence that hemi-nested PCR protocols are prone to the development of artifacts consisting of multiple head-to-tail primer tandem repeats is presented. This phenomenon was more pronounced with the addition of a large amount of template into the second round PCR and was limited to the primer used in both first and second reactions.

Original language	English (US)
Pages (from-to)	385-387
Number of pages	3
Journal	Molecular and Cellular Probes
Volume	11
Issue number	5
DOIs	https://doi.org/10.1006/mcpr.1997.0122
State	Published - Oct 1997

Keywords

Artifact
PCR
Primer repeats

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1006/mcpr.1997.0122

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title = "Head-to-tail primer tandem repeats generated in hemi-nested PCR",

abstract = "Evidence that hemi-nested PCR protocols are prone to the development of artifacts consisting of multiple head-to-tail primer tandem repeats is presented. This phenomenon was more pronounced with the addition of a large amount of template into the second round PCR and was limited to the primer used in both first and second reactions.",

keywords = "Artifact, PCR, Primer repeats",

author = "Wang, {L. F.} and J. Rakela and T. Laskus",

year = "1997",

month = oct,

doi = "10.1006/mcpr.1997.0122",

language = "English (US)",

volume = "11",

pages = "385--387",

journal = "Molecular and Cellular Probes",

issn = "0890-8508",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Head-to-tail primer tandem repeats generated in hemi-nested PCR

AU - Wang, L. F.

AU - Rakela, J.

AU - Laskus, T.

PY - 1997/10

Y1 - 1997/10

N2 - Evidence that hemi-nested PCR protocols are prone to the development of artifacts consisting of multiple head-to-tail primer tandem repeats is presented. This phenomenon was more pronounced with the addition of a large amount of template into the second round PCR and was limited to the primer used in both first and second reactions.

AB - Evidence that hemi-nested PCR protocols are prone to the development of artifacts consisting of multiple head-to-tail primer tandem repeats is presented. This phenomenon was more pronounced with the addition of a large amount of template into the second round PCR and was limited to the primer used in both first and second reactions.

KW - Artifact

KW - PCR

KW - Primer repeats

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U2 - 10.1006/mcpr.1997.0122

DO - 10.1006/mcpr.1997.0122

M3 - Article

C2 - 9375299

AN - SCOPUS:0031260240

SN - 0890-8508

VL - 11

SP - 385

EP - 387

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

IS - 5

ER -

Head-to-tail primer tandem repeats generated in hemi-nested PCR

Abstract

Keywords

ASJC Scopus subject areas

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