Head-to-tail primer tandem repeats generated in hemi-nested PCR

L. F. Wang, Jorge Rakela, T. Laskus

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Evidence that hemi-nested PCR protocols are prone to the development of artifacts consisting of multiple head-to-tail primer tandem repeats is presented. This phenomenon was more pronounced with the addition of a large amount of template into the second round PCR and was limited to the primer used in both first and second reactions.

Original languageEnglish (US)
Pages (from-to)385-387
Number of pages3
JournalMolecular and Cellular Probes
Volume11
Issue number5
DOIs
StatePublished - Oct 1997
Externally publishedYes

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Keywords

  • Artifact
  • PCR
  • Primer repeats

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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