H/D exchange centroid monitoring is insufficient to show differences in the behavior of protein states

Jun Zhang, Pradeep Ramachandran, Rajiv Kumar, Michael L. Gross

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Differential hydrogen/deuterium exchange (H/DX) coupled with mass spectrometry (H/DX-MS) offers a rapid and sensitive characterization of changes in proteins following perturbations induced by changes in folding, ligand binding, oligomerization, and modification. The characterization of H/DX rates by software tools and automated data processing often relies on the centroid mass calculation and, thereby, the deuterium distribution in the mass spectra is neglected. Here we present an example demonstrating the clear limitation of using only a centroid approach to characterize the H/DX rate, in which the change in protein is not reflected as the difference in deuterium uptake based on centroid calculation.

Original languageEnglish (US)
Pages (from-to)450-453
Number of pages4
JournalJournal of the American Society for Mass Spectrometry
Volume24
Issue number3
DOIs
StatePublished - Mar 1 2013

Keywords

  • Centroid of D distribution
  • DREAM protein
  • EX1 and EX2 kinetics
  • Hydrogen/deuterium exchange
  • Mass spectrometry

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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