Harpagophytum procumbens suppresses lipopolysaccharide-stimulated expressions of cyclooxygenase-2 and inducible nitric oxide synthase in fibroblast cell line L929

Mi-Hyeon D Jang, Sabina Lim, Seung Moo Han, Hi Joon Park, Insop Shin, Jin Woo Kim, Nam Jae Kim, Ji Suk Lee, Kyung Ah Kim, Chang Ju Kim

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Harpagophytum procumbens (Pedaliaceae) has been used for the treatment of pain and arthritis. The effect of Harpagophytum procumbens against lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E2 (PGE2) immunoassay, and nitric oxide detection on mouse fibroblast cell line L929. The aqueous extract of Harpagophytum procumbens was shown to suppress PGE2 synthesis and nitric oxide production by inhibiting lipopolysaccharide-stimulated enhancement of the cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) mRNAs expressions in L929 cells. These results suggest that Harpagophytum procumbens exerts anti-inflammatory and analgesic effects probably by suppressing cyclooxygenase-2 and iNOS expressions.

Original languageEnglish (US)
Pages (from-to)367-371
Number of pages5
JournalJournal of Pharmacological Sciences
Volume93
Issue number3
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Harpagophytum
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Lipopolysaccharides
Fibroblasts
Cell Line
Dinoprostone
Pedaliaceae
Nitric Oxide
Non-Steroidal Anti-Inflammatory Agents
Immunoassay
Reverse Transcription
Arthritis
Inflammation
Pain
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cyclooxygenase-2
  • Harpagophytum procumbens
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Pharmacology

Cite this

Harpagophytum procumbens suppresses lipopolysaccharide-stimulated expressions of cyclooxygenase-2 and inducible nitric oxide synthase in fibroblast cell line L929. / Jang, Mi-Hyeon D; Lim, Sabina; Han, Seung Moo; Park, Hi Joon; Shin, Insop; Kim, Jin Woo; Kim, Nam Jae; Lee, Ji Suk; Kim, Kyung Ah; Kim, Chang Ju.

In: Journal of Pharmacological Sciences, Vol. 93, No. 3, 11.2003, p. 367-371.

Research output: Contribution to journalArticle

Jang, Mi-Hyeon D ; Lim, Sabina ; Han, Seung Moo ; Park, Hi Joon ; Shin, Insop ; Kim, Jin Woo ; Kim, Nam Jae ; Lee, Ji Suk ; Kim, Kyung Ah ; Kim, Chang Ju. / Harpagophytum procumbens suppresses lipopolysaccharide-stimulated expressions of cyclooxygenase-2 and inducible nitric oxide synthase in fibroblast cell line L929. In: Journal of Pharmacological Sciences. 2003 ; Vol. 93, No. 3. pp. 367-371.
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