Harpagophytum procumbens suppresses lipopolysaccharide-stimulated expressions of cyclooxygenase-2 and inducible nitric oxide synthase in fibroblast cell line L929

Mi Hyeon Jang, Sabina Lim, Seung Moo Han, Hi Joon Park, Insop Shin, Jin Woo Kim, Nam Jae Kim, Ji Suk Lee, Kyung Ah Kim, Chang Ju Kim

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Harpagophytum procumbens (Pedaliaceae) has been used for the treatment of pain and arthritis. The effect of Harpagophytum procumbens against lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E2 (PGE2) immunoassay, and nitric oxide detection on mouse fibroblast cell line L929. The aqueous extract of Harpagophytum procumbens was shown to suppress PGE2 synthesis and nitric oxide production by inhibiting lipopolysaccharide-stimulated enhancement of the cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) mRNAs expressions in L929 cells. These results suggest that Harpagophytum procumbens exerts anti-inflammatory and analgesic effects probably by suppressing cyclooxygenase-2 and iNOS expressions.

Original languageEnglish (US)
Pages (from-to)367-371
Number of pages5
JournalJournal of Pharmacological Sciences
Issue number3
StatePublished - Nov 1 2003



  • Cyclooxygenase-2
  • Harpagophytum procumbens
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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