Haploinsufficiency of mechanistic target of rapamycin ameliorates bag3 cardiomyopathy in adult zebrafish

Yonghe Ding, Alexey V. Dvornikov, Xiao Ma, Hong Zhang, Yong Wang, Matthew Lowerison, Rene R. Packard, Lei Wang, Jun Chen, Yuji Zhang, Tzung Hsiai, Xueying Lin, Xiaolei Xu

Research output: Contribution to journalArticle

Abstract

The adult zebrafish is an emerging vertebrate model for studying human cardiomyopathies; however, whether the simple zebrafish heart can model different subtypes of cardiomyopathies, such as dilated cardiomyopathy (DCM), remains elusive. Here, we generated and characterized an inherited DCM model in adult zebrafish and used this model to search for therapeutic strategies. We employed transcription activator-like effector nuclease (TALEN) genome editing technology to generate frame-shift mutants for the zebrafish ortholog of human BCL2-associated athanogene 3 (BAG3), an established DCM-causative gene. As in mammals, the zebrafish bag3 homozygous mutant (bag3e2/e2 ) exhibited aberrant proteostasis, as indicated by impaired autophagy flux and elevated ubiquitinated protein aggregation. Through comprehensive phenotyping analysis of the mutant, we identified phenotypic traits that resembled DCM phenotypes in mammals, including cardiac chamber enlargement, reduced ejection fraction characterized by increased end-systolic volume/body weight (ESV/BW), and reduced contractile myofibril activation kinetics. Nonbiased transcriptome analysis identified the hyperactivation of the mechanistic target of rapamycin (mTOR) signaling in bag3e2/e2 mutant hearts. Further genetic studies showed that mtorxu015/+ , an mTOR haploinsufficiency mutant, repaired abnormal proteostasis, improved cardiac function and rescued the survival of the bag3e2/e2 mutant. This study established the bag3e2/e2 mutant as a DCM model in adult zebrafish and suggested mtor as a candidate therapeutic target gene for BAG3 cardiomyopathy.

Original languageEnglish (US)
JournalDisease models & mechanisms
Volume12
Issue number10
DOIs
StatePublished - Oct 1 2019

Fingerprint

Haploinsufficiency
Zebrafish
Sirolimus
Cardiomyopathies
Dilated Cardiomyopathy
Mammals
Genes
Ubiquitinated Proteins
Transcription
Myofibrils
Autophagy
Gene Expression Profiling
Agglomeration
Chemical activation
Vertebrates
Fluxes
Body Weight
Kinetics
Technology
Phenotype

Keywords

  • BCL2-associated athanogene 3
  • Danio rerio
  • Dilated cardiomyopathy
  • mTOR

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Haploinsufficiency of mechanistic target of rapamycin ameliorates bag3 cardiomyopathy in adult zebrafish. / Ding, Yonghe; Dvornikov, Alexey V.; Ma, Xiao; Zhang, Hong; Wang, Yong; Lowerison, Matthew; Packard, Rene R.; Wang, Lei; Chen, Jun; Zhang, Yuji; Hsiai, Tzung; Lin, Xueying; Xu, Xiaolei.

In: Disease models & mechanisms, Vol. 12, No. 10, 01.10.2019.

Research output: Contribution to journalArticle

Ding, Y, Dvornikov, AV, Ma, X, Zhang, H, Wang, Y, Lowerison, M, Packard, RR, Wang, L, Chen, J, Zhang, Y, Hsiai, T, Lin, X & Xu, X 2019, 'Haploinsufficiency of mechanistic target of rapamycin ameliorates bag3 cardiomyopathy in adult zebrafish', Disease models & mechanisms, vol. 12, no. 10. https://doi.org/10.1242/dmm.040154
Ding, Yonghe ; Dvornikov, Alexey V. ; Ma, Xiao ; Zhang, Hong ; Wang, Yong ; Lowerison, Matthew ; Packard, Rene R. ; Wang, Lei ; Chen, Jun ; Zhang, Yuji ; Hsiai, Tzung ; Lin, Xueying ; Xu, Xiaolei. / Haploinsufficiency of mechanistic target of rapamycin ameliorates bag3 cardiomyopathy in adult zebrafish. In: Disease models & mechanisms. 2019 ; Vol. 12, No. 10.
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AU - Lowerison, Matthew

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