Human tissue for flow cytometry must be prepared as an adequate single-cell suspension. The appropriate methods for tissue collection, transport, storage, and dissociation depend on the cell parameters being measured and the localization of the markers. This unit includes a general method for collecting and transporting human tissue and preparing a tissue imprint. Protocols are supplied for tissue disaggregation by either mechanical or enzymatic means and for preparation of single-cell suspensions of whole cells from fine-needle aspirates, pleural effusions, abdominal fluids, or other body fluids. Other protocols detail preparation of intact nuclei from fresh, frozen, or paraffin-embedded tissue. Support protocols cover fixation, cryospin preparation, cryopreservation, and removal of debris.
|Original language||English (US)|
|Pages (from-to)||Unit 5.2|
|Journal||Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]|
|State||Published - May 2001|
ASJC Scopus subject areas
- Medical Laboratory Technology