Handling, storage, and preparation of human tissues.

L. G. Dressler, D. Visscher

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Human tissue for flow cytometry must be prepared as an adequate single-cell suspension. The appropriate methods for tissue collection, transport, storage, and dissociation depend on the cell parameters being measured and the localization of the markers. This unit includes a general method for collecting and transporting human tissue and preparing a tissue imprint. Protocols are supplied for tissue disaggregation by either mechanical or enzymatic means and for preparation of single-cell suspensions of whole cells from fine-needle aspirates, pleural effusions, abdominal fluids, or other body fluids. Other protocols detail preparation of intact nuclei from fresh, frozen, or paraffin-embedded tissue. Support protocols cover fixation, cryospin preparation, cryopreservation, and removal of debris.

Original languageEnglish (US)
Pages (from-to)Unit 5.2
JournalCurrent protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]
VolumeChapter 5
StatePublished - May 2001

ASJC Scopus subject areas

  • Biochemistry
  • Histology
  • Medical Laboratory Technology

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