TY - JOUR
T1 - Handling, storage, and preparation of human tissues.
AU - Dressler, L. G.
AU - Visscher, D.
PY - 2001/5
Y1 - 2001/5
N2 - Human tissue for flow cytometry must be prepared as an adequate single-cell suspension. The appropriate methods for tissue collection, transport, storage, and dissociation depend on the cell parameters being measured and the localization of the markers. This unit includes a general method for collecting and transporting human tissue and preparing a tissue imprint. Protocols are supplied for tissue disaggregation by either mechanical or enzymatic means and for preparation of single-cell suspensions of whole cells from fine-needle aspirates, pleural effusions, abdominal fluids, or other body fluids. Other protocols detail preparation of intact nuclei from fresh, frozen, or paraffin-embedded tissue. Support protocols cover fixation, cryospin preparation, cryopreservation, and removal of debris.
AB - Human tissue for flow cytometry must be prepared as an adequate single-cell suspension. The appropriate methods for tissue collection, transport, storage, and dissociation depend on the cell parameters being measured and the localization of the markers. This unit includes a general method for collecting and transporting human tissue and preparing a tissue imprint. Protocols are supplied for tissue disaggregation by either mechanical or enzymatic means and for preparation of single-cell suspensions of whole cells from fine-needle aspirates, pleural effusions, abdominal fluids, or other body fluids. Other protocols detail preparation of intact nuclei from fresh, frozen, or paraffin-embedded tissue. Support protocols cover fixation, cryospin preparation, cryopreservation, and removal of debris.
UR - http://www.scopus.com/inward/record.url?scp=52249092406&partnerID=8YFLogxK
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M3 - Article
C2 - 18770712
AN - SCOPUS:52249092406
SN - 1934-9297
VL - Chapter 5
SP - Unit 5.2
JO - Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]
JF - Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]
ER -