HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells

Jaime O. Claudio, Yuan Xiao Zhu, Sally J. Benn, Anu Heidi Shukla, C. Jane McGlade, Nathan Falcioni, A. Keith Stewart

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

SH3 and SAM domains are protein interaction motifs that are predominantly seen in signaling molecules, adaptors, and scaffold proteins. We have identified a novel family of putative adaptor genes that includes HACS1. HACS1 encodes a 441 amino acid protein that is differentially expressed in hematopoietic cells and has restricted expression in human tissues. Its SH3 domain is most similar to the same motif in Crk and its SAM domain shares homology with a family of uncharacterized putative scaffold and adaptor proteins. HACS1 maps to human chromosome 21q11.2 in a region that is frequently disrupted by translocation events in hematopoietic malignancies. Polyclonal antibodies against HACS1 recognized a 49.5 kDa protein whose mRNA is expressed in human immune tissues, bone marrow, heart, lung, placenta and brain. Cell lines and primary cells from acute myeloid leukemias and multiple myeloma patients express HACS1. Immunostaining and cellular fractionation studies localized the HACS1 protein predominantly to the cytoplasm.

Original languageEnglish (US)
Pages (from-to)5373-5377
Number of pages5
JournalOncogene
Volume20
Issue number38
DOIs
StatePublished - Aug 30 2001

Keywords

  • Adaptor protein
  • Hematopoietic
  • Myeloma
  • SAM domain
  • SH3 domain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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