H-2 effects on cell-cell interactions in the response to single non-H-2 alloantigens. IV. Variation in the proliferative responses to H-Y and H-3

P. J. Wettstein

Research output: Contribution to journalArticle

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Abstract

Individual mice were tested for their proliferative T-cell response to H-Y- and H-3-incompatible stimulator cells in secondary mixed lymphocyte culture. Responders expressing the H-2(b) haplotype were restricted in their response to stimulators presenting H-Y and H-3 in the context of H-2(b). Lymphocytes from individual B10 females proliferated in response to H-Y presented with I-A(b) and D(b). The ratio of I-A(b)/D(b)-restricted responses varied between individual responders, indicating significant qualitative variation between genetically identical responders. The majority of the proliferative response in all tested mice was restricted to the entire H-2(b) haplotype suggesting complementation of I-A(b)- and D(b)-region genes in presenting the H-Y antigen. Similar observations were made in the response of individual B10.LP mice to the H-3 antigen. H-3-specific, proliferating T cells were restricted to H-3 antigen presented with K(b)A(b) and D(b) with significant variation between individual in their preference for H-3 plus K(b)A(b) and D(b). In contrast to the response to H-Y, the proliferative response to H-3 plus H-2(b) could be accounted for by the summation of the proliferative responses to H-3 plus K(b)A(b) and D(b). These observations demonstrate that the proliferative response to non-H-2H antigens in the context of I-region determinants is not a sine qua non for the T-cell response to these antigens. Further, the individual qualitative and quantitative variation observed with individual genetically identical mice has strong implications for our knowledge of intrastrain variation in immune responsiveness and the characterization of inbred strains for immune responsiveness.

Original languageEnglish (US)
Pages (from-to)241-252
Number of pages12
JournalImmunogenetics
Volume14
Issue number3-4
StatePublished - 1981
Externally publishedYes

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Isoantigens
Cell Communication
T-Lymphocytes
Haplotypes
H-Y Antigen
Lymphocytes
Antigens
Genes
H antigen

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

H-2 effects on cell-cell interactions in the response to single non-H-2 alloantigens. IV. Variation in the proliferative responses to H-Y and H-3. / Wettstein, P. J.

In: Immunogenetics, Vol. 14, No. 3-4, 1981, p. 241-252.

Research output: Contribution to journalArticle

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