Growth properties of a feline immunodeficiency virus mutant which lacks an AP-1 binding site in primary peripheral blood mononuclear cells.

T. Miyazawa, Y. Inoshima, M. Kohmoto, Yasuhiro H Ikeda, T. Mikami

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Thirty-one base pairs (bp) containing putative AP-1 and AP-4 binding sequences in the U3 region of feline immunodeficiency virus (FIV) long terminal repeat (LTR) were deleted from an infectious molecular clone of FIV for construction of a mutant virus, and the replication rate and the cytopathogenic activity of the virus were compared with those of the wild type virus in concanavalin-A (Con-A) stimulated primary feline peripheral blood mononuclear cells (fPBMCs). It was found that the replication rate and cytopathogenic activity of the mutant were almost the same as those of the wild type. The deletion of the mutant virus was stable during the infection experiments. From these data, we concluded that the 31 bp fragment in the LTR is not required for the replication of FIV in Con-A stimulated primary fPBMC.

Original languageEnglish (US)
Pages (from-to)869-872
Number of pages4
JournalThe Journal of veterinary medical science / the Japanese Society of Veterinary Science
Volume56
Issue number5
StatePublished - Oct 1994
Externally publishedYes

Fingerprint

Feline Immunodeficiency Virus
Feline immunodeficiency virus
Transcription Factor AP-1
mononuclear leukocytes
binding sites
Blood Cells
Terminal Repeat Sequences
Binding Sites
Felidae
Concanavalin A
Viruses
Base Pairing
viruses
mutants
terminal repeat sequences
concanavalin A
Growth
cats
Virus Replication
Clone Cells

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

Cite this

Growth properties of a feline immunodeficiency virus mutant which lacks an AP-1 binding site in primary peripheral blood mononuclear cells. / Miyazawa, T.; Inoshima, Y.; Kohmoto, M.; Ikeda, Yasuhiro H; Mikami, T.

In: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 56, No. 5, 10.1994, p. 869-872.

Research output: Contribution to journalArticle

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