Growth hormone and growth hormone-related mRNA in uremic rats

Effect of a growth hormone secretagogue

Richard J. Krieg, Winnie Chan, Kwei C. Lin, Nancy B. Kuemmerle, Johannes D Veldhuis, J. C M Chan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In experimental animals, the decreased growth during mild uremia is not accompanied by a loss in the capacity of the pituitary gland to secrete growth hormone (GH). With the development of orally administered GH "secretagogues" (GHS), it might be possible to stimulate growth during uremia without injections. This study was designed to determine the effects of the GHS, L-163,255. Uremia was induced by 5/6 nephrectomy (NX). GHS was given orally, 3 mg/kg, twice a week. Four groups of animals included: (1) sham-operated, (2) sham-operated, pair-fed, (3) uremic (NX), and (4) uremic, GHS-treated (NX+GHS). Blood sampling was conducted via intra-atrial catheters, and GH was quantitated. Pituitary GH mRNA was measured by Northern blot, and liver GH receptor and insulin-like growth factor-I mRNAs by RNAase protection. Untreated NX animals had a specific decrease in the massof the GH pulses. A burst of GH was induced by GHS, but the pulsatile pattern of GH secretion over 6 h was not affected. An increase or a return to non-uremic levels of GH-related mRNAs occurred after GHS. Thus, GHS stimulated an acute burst of GH secretion and increased specific mRNAs encoding GH-related proteins in uremic animals.

Original languageEnglish (US)
Pages (from-to)585-590
Number of pages6
JournalPediatric Nephrology
Volume17
Issue number8
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Growth Hormone
Messenger RNA
Uremia
L 163255
Somatotropin Receptors
Pituitary Gland
Growth
Nephrectomy
Insulin-Like Growth Factor I
Northern Blotting
Catheters
Injections
Liver

Keywords

  • Growth hormone
  • Growth hormone receptor
  • Insulin-like growth factor
  • mRNA
  • Partial nephrectomy
  • Pituitary, growth hormone secretagogue

ASJC Scopus subject areas

  • Nephrology
  • Pediatrics, Perinatology, and Child Health

Cite this

Krieg, R. J., Chan, W., Lin, K. C., Kuemmerle, N. B., Veldhuis, J. D., & Chan, J. C. M. (2002). Growth hormone and growth hormone-related mRNA in uremic rats: Effect of a growth hormone secretagogue. Pediatric Nephrology, 17(8), 585-590. https://doi.org/10.1007/s00467-002-0893-3

Growth hormone and growth hormone-related mRNA in uremic rats : Effect of a growth hormone secretagogue. / Krieg, Richard J.; Chan, Winnie; Lin, Kwei C.; Kuemmerle, Nancy B.; Veldhuis, Johannes D; Chan, J. C M.

In: Pediatric Nephrology, Vol. 17, No. 8, 2002, p. 585-590.

Research output: Contribution to journalArticle

Krieg, RJ, Chan, W, Lin, KC, Kuemmerle, NB, Veldhuis, JD & Chan, JCM 2002, 'Growth hormone and growth hormone-related mRNA in uremic rats: Effect of a growth hormone secretagogue', Pediatric Nephrology, vol. 17, no. 8, pp. 585-590. https://doi.org/10.1007/s00467-002-0893-3
Krieg, Richard J. ; Chan, Winnie ; Lin, Kwei C. ; Kuemmerle, Nancy B. ; Veldhuis, Johannes D ; Chan, J. C M. / Growth hormone and growth hormone-related mRNA in uremic rats : Effect of a growth hormone secretagogue. In: Pediatric Nephrology. 2002 ; Vol. 17, No. 8. pp. 585-590.
@article{a30678062f4e4d26a1e4d3751dc20052,
title = "Growth hormone and growth hormone-related mRNA in uremic rats: Effect of a growth hormone secretagogue",
abstract = "In experimental animals, the decreased growth during mild uremia is not accompanied by a loss in the capacity of the pituitary gland to secrete growth hormone (GH). With the development of orally administered GH {"}secretagogues{"} (GHS), it might be possible to stimulate growth during uremia without injections. This study was designed to determine the effects of the GHS, L-163,255. Uremia was induced by 5/6 nephrectomy (NX). GHS was given orally, 3 mg/kg, twice a week. Four groups of animals included: (1) sham-operated, (2) sham-operated, pair-fed, (3) uremic (NX), and (4) uremic, GHS-treated (NX+GHS). Blood sampling was conducted via intra-atrial catheters, and GH was quantitated. Pituitary GH mRNA was measured by Northern blot, and liver GH receptor and insulin-like growth factor-I mRNAs by RNAase protection. Untreated NX animals had a specific decrease in the massof the GH pulses. A burst of GH was induced by GHS, but the pulsatile pattern of GH secretion over 6 h was not affected. An increase or a return to non-uremic levels of GH-related mRNAs occurred after GHS. Thus, GHS stimulated an acute burst of GH secretion and increased specific mRNAs encoding GH-related proteins in uremic animals.",
keywords = "Growth hormone, Growth hormone receptor, Insulin-like growth factor, mRNA, Partial nephrectomy, Pituitary, growth hormone secretagogue",
author = "Krieg, {Richard J.} and Winnie Chan and Lin, {Kwei C.} and Kuemmerle, {Nancy B.} and Veldhuis, {Johannes D} and Chan, {J. C M}",
year = "2002",
doi = "10.1007/s00467-002-0893-3",
language = "English (US)",
volume = "17",
pages = "585--590",
journal = "Pediatric nephrology (Berlin, Germany)",
issn = "0931-041X",
publisher = "Springer Verlag",
number = "8",

}

TY - JOUR

T1 - Growth hormone and growth hormone-related mRNA in uremic rats

T2 - Effect of a growth hormone secretagogue

AU - Krieg, Richard J.

AU - Chan, Winnie

AU - Lin, Kwei C.

AU - Kuemmerle, Nancy B.

AU - Veldhuis, Johannes D

AU - Chan, J. C M

PY - 2002

Y1 - 2002

N2 - In experimental animals, the decreased growth during mild uremia is not accompanied by a loss in the capacity of the pituitary gland to secrete growth hormone (GH). With the development of orally administered GH "secretagogues" (GHS), it might be possible to stimulate growth during uremia without injections. This study was designed to determine the effects of the GHS, L-163,255. Uremia was induced by 5/6 nephrectomy (NX). GHS was given orally, 3 mg/kg, twice a week. Four groups of animals included: (1) sham-operated, (2) sham-operated, pair-fed, (3) uremic (NX), and (4) uremic, GHS-treated (NX+GHS). Blood sampling was conducted via intra-atrial catheters, and GH was quantitated. Pituitary GH mRNA was measured by Northern blot, and liver GH receptor and insulin-like growth factor-I mRNAs by RNAase protection. Untreated NX animals had a specific decrease in the massof the GH pulses. A burst of GH was induced by GHS, but the pulsatile pattern of GH secretion over 6 h was not affected. An increase or a return to non-uremic levels of GH-related mRNAs occurred after GHS. Thus, GHS stimulated an acute burst of GH secretion and increased specific mRNAs encoding GH-related proteins in uremic animals.

AB - In experimental animals, the decreased growth during mild uremia is not accompanied by a loss in the capacity of the pituitary gland to secrete growth hormone (GH). With the development of orally administered GH "secretagogues" (GHS), it might be possible to stimulate growth during uremia without injections. This study was designed to determine the effects of the GHS, L-163,255. Uremia was induced by 5/6 nephrectomy (NX). GHS was given orally, 3 mg/kg, twice a week. Four groups of animals included: (1) sham-operated, (2) sham-operated, pair-fed, (3) uremic (NX), and (4) uremic, GHS-treated (NX+GHS). Blood sampling was conducted via intra-atrial catheters, and GH was quantitated. Pituitary GH mRNA was measured by Northern blot, and liver GH receptor and insulin-like growth factor-I mRNAs by RNAase protection. Untreated NX animals had a specific decrease in the massof the GH pulses. A burst of GH was induced by GHS, but the pulsatile pattern of GH secretion over 6 h was not affected. An increase or a return to non-uremic levels of GH-related mRNAs occurred after GHS. Thus, GHS stimulated an acute burst of GH secretion and increased specific mRNAs encoding GH-related proteins in uremic animals.

KW - Growth hormone

KW - Growth hormone receptor

KW - Insulin-like growth factor

KW - mRNA

KW - Partial nephrectomy

KW - Pituitary, growth hormone secretagogue

UR - http://www.scopus.com/inward/record.url?scp=0036956993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036956993&partnerID=8YFLogxK

U2 - 10.1007/s00467-002-0893-3

DO - 10.1007/s00467-002-0893-3

M3 - Article

VL - 17

SP - 585

EP - 590

JO - Pediatric nephrology (Berlin, Germany)

JF - Pediatric nephrology (Berlin, Germany)

SN - 0931-041X

IS - 8

ER -