Growth hormone and growth hormone-related mRNA in uremic rats: Effect of a growth hormone secretagogue

Richard J. Krieg, Winnie Chan, Kwei Chi Lin, Nancy B. Kuemmerle, Johannes D. Veldhuis, James C.M. Chan

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

In experimental animals, the decreased growth during mild uremia is not accompanied by a loss in the capacity of the pituitary gland to secrete growth hormone (GH). With the development of orally administered GH "secretagogues" (GHS), it might be possible to stimulate growth during uremia without injections. This study was designed to determine the effects of the GHS, L-163,255. Uremia was induced by 5/6 nephrectomy (NX). GHS was given orally, 3 mg/kg, twice a week. Four groups of animals included: (1) sham-operated, (2) sham-operated, pair-fed, (3) uremic (NX), and (4) uremic, GHS-treated (NX+GHS). Blood sampling was conducted via intra-atrial catheters, and GH was quantitated. Pituitary GH mRNA was measured by Northern blot, and liver GH receptor and insulin-like growth factor-I mRNAs by RNAase protection. Untreated NX animals had a specific decrease in the mass of the GH pulses. A burst of GH was induced by GHS, but the pulsatile pattern of GH secretion over 6 h was not affected. An increase or a return to non-uremic levels of GH-related mRNAs occurred after GHS. Thus, GHS stimulated an acute burst of GH secretion and increased specific mRNAs encoding GH-related proteins in uremic animals.

Original languageEnglish (US)
Pages (from-to)585-590
Number of pages6
JournalPediatric Nephrology
Volume17
Issue number8
DOIs
StatePublished - Dec 1 2002

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Keywords

  • Growth hormone
  • Growth hormone receptor
  • Insulin-like growth factor
  • Partial nephrectomy
  • Pituitary, growth hormone secretagogue
  • mRNA

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Nephrology

Cite this

Krieg, R. J., Chan, W., Lin, K. C., Kuemmerle, N. B., Veldhuis, J. D., & Chan, J. C. M. (2002). Growth hormone and growth hormone-related mRNA in uremic rats: Effect of a growth hormone secretagogue. Pediatric Nephrology, 17(8), 585-590. https://doi.org/10.1007/s00467-002-0893-3