Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils

Hirohito Kita, Tsukasa Ohnishi, Yoshio Okubo, Deborah Weiler, John S. Abrams, Gerald J. Gleich

Research output: Contribution to journalArticlepeer-review

257 Scopus citations

Abstract

Human peripheral blood eosinophils released eosinophil survival-enhancing activity when stimulated with the calcium ionophore, ionomycin. The release of activity was detected as early as 3 h after stimulation and was inhibited by an immunomodulating agent, cyclosporin A. The survivalenhancing activity was completely abolished by treatment with anti-interleukin 3 (IL3) and anti-granulocyte/macrophage colony-stimulating factor (GM-CSF) monoclonal antibodies. Moreover, IL-3 and GM-CSF were measurable in ionomycin-stimulated eosinophil supernatants by immunoassay. Eosinophils produced approximately one-half as much IL3 and one-fifth as much GM-CSF as ionomycin-stimulated mononuclear cells. Neutrophils also produced 11,3 and GM-CSF, but the amounts were less than those produced by eosinophils. These observations suggest a novel role for eosinophils in pathophysiology of allergic inflammation and host defense mechanisms.

Original languageEnglish (US)
Pages (from-to)745-748
Number of pages4
JournalJournal of Experimental Medicine
Volume174
Issue number3
DOIs
StatePublished - Sep 1 1991

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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