Granulocyte macrophage colony-stimulating factor augments ICAM-1 and VCAM-1 activation of eosinophil function

Makoto Nagata, Julie B. Sedgwick, Hirohito Kita, William W. Busse

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are members of the immunoglobulin superfamily adhesion molecules on vascular endothelium and important in the development of eosinophil (EOS) accumulation in allergic inflammation. To define the role of these adhesion proteins in EOS inflammation, peripheral blood EOS from allergic donors were incubated in either buffer (control)-, recombinant human (rh)-VCAM-1-, or rh-ICAM-1-coated plates, and the effects of these adhesion proteins on EOS effector functions were determined. VCAM-1 induced spontaneous EOS adhesion whereas EOS adhesion to ICAM-1 required a second signal, such as granulocyte macrophage colony-stimulating factor (GM-CSF). Although only VCAM-1 stimulated EOS superoxide anion (O2) generation, the addition of GM-CSF (100 pM) to the reactions resulted in a greater and equivalent production of O2 with VCAM-1 and ICAM-1. In the presence of GM-CSE, ICAM-1 and VCAM-1 caused significant release of EOS-derived neurotoxin (EDN). Moreover, only ICAM-1 (no GM-CSF) promoted calcium ionophore A23187 (0.2 μM)-induced EOS leukotriene C4 (LTC4). Enhanced O2 generation, EDN release, and LTC4 generation observed with ICAM-1 and VCAM-1 were significantly inhibited by anti-β2-integrin antibody. These results suggest that ICAM-1 and VCAM-1 are important in determining the eventual function of airway EOS.

Original languageEnglish (US)
Pages (from-to)158-166
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume19
Issue number1
StatePublished - 1998

Fingerprint

Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Granulocyte-Macrophage Colony-Stimulating Factor
Eosinophils
Chemical activation
Adhesion
Leukotriene C4
Eosinophil-Derived Neurotoxin
Inflammation
Calcium Ionophores
Neurotoxins
Vascular Endothelium
Calcimycin
Integrins
Superoxides
Immunoglobulins
Buffers
Proteins
Blood
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Granulocyte macrophage colony-stimulating factor augments ICAM-1 and VCAM-1 activation of eosinophil function. / Nagata, Makoto; Sedgwick, Julie B.; Kita, Hirohito; Busse, William W.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 19, No. 1, 1998, p. 158-166.

Research output: Contribution to journalArticle

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