Glycoxidation of Low-Density Lipoprotein Increases TUNEL Positivity and CPP32 Activation in Human Coronary Cells

Filomena De Nigris; Gianfranco Tajana; Mario Condorelli; Francesco P. D'Armiento; Giacomo Sica; Lilach O. Lerman; Claudio Napoli

doi:10.1196/annals.1299.128

Glycoxidation of Low-Density Lipoprotein Increases TUNEL Positivity and CPP32 Activation in Human Coronary Cells

Filomena De Nigris, Gianfranco Tajana, Mario Condorelli, Francesco P. D'Armiento, Giacomo Sica, Lilach O. Lerman, Claudio Napoli

Nephrology and Hypertension

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Apoptosis of arterial cells induced by oxidized low-density lipoprotein (oxLDL) is thought to contribute to the progression of vascular dysfunction and atherogenesis. It is well established that diabetes mellitus is accompanied by both glycosylation and oxidation of LDL (glc-oxLDL), but the biological effects of these modified lipoproteins are poorly understood. We demonstrate here for the first time that glc-oxLDL increases TUNEL positivity and caspase-3 activation (by Western blot and immunocytochemistry) of human coronary smooth muscle cells. Overall, these effects induced by glc-oxLDL were greater than those achieved with oxLDL. Thus, glc-oxLDL activated downstream apoptotic signaling. This may influence the evolution of atherogenesis and vascular complications in diabetes.

Original language	English (US)
Pages (from-to)	710-715
Number of pages	6
Journal	Annals of the New York Academy of Sciences
Volume	1010
DOIs	https://doi.org/10.1196/annals.1299.128
State	Published - 2003

Keywords

Atherosclerosis
Caspase
glc-oxLDL
oxLDL

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
History and Philosophy of Science

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10.1196/annals.1299.128

Cite this

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title = "Glycoxidation of Low-Density Lipoprotein Increases TUNEL Positivity and CPP32 Activation in Human Coronary Cells",

abstract = "Apoptosis of arterial cells induced by oxidized low-density lipoprotein (oxLDL) is thought to contribute to the progression of vascular dysfunction and atherogenesis. It is well established that diabetes mellitus is accompanied by both glycosylation and oxidation of LDL (glc-oxLDL), but the biological effects of these modified lipoproteins are poorly understood. We demonstrate here for the first time that glc-oxLDL increases TUNEL positivity and caspase-3 activation (by Western blot and immunocytochemistry) of human coronary smooth muscle cells. Overall, these effects induced by glc-oxLDL were greater than those achieved with oxLDL. Thus, glc-oxLDL activated downstream apoptotic signaling. This may influence the evolution of atherogenesis and vascular complications in diabetes.",

keywords = "Atherosclerosis, Caspase, glc-oxLDL, oxLDL",

author = "{De Nigris}, Filomena and Gianfranco Tajana and Mario Condorelli and D'Armiento, {Francesco P.} and Giacomo Sica and Lerman, {Lilach O.} and Claudio Napoli",

year = "2003",

doi = "10.1196/annals.1299.128",

language = "English (US)",

volume = "1010",

pages = "710--715",

journal = "Annals of the New York Academy of Sciences",

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T1 - Glycoxidation of Low-Density Lipoprotein Increases TUNEL Positivity and CPP32 Activation in Human Coronary Cells

AU - De Nigris, Filomena

AU - Tajana, Gianfranco

AU - Condorelli, Mario

AU - D'Armiento, Francesco P.

AU - Sica, Giacomo

AU - Lerman, Lilach O.

AU - Napoli, Claudio

PY - 2003

Y1 - 2003

N2 - Apoptosis of arterial cells induced by oxidized low-density lipoprotein (oxLDL) is thought to contribute to the progression of vascular dysfunction and atherogenesis. It is well established that diabetes mellitus is accompanied by both glycosylation and oxidation of LDL (glc-oxLDL), but the biological effects of these modified lipoproteins are poorly understood. We demonstrate here for the first time that glc-oxLDL increases TUNEL positivity and caspase-3 activation (by Western blot and immunocytochemistry) of human coronary smooth muscle cells. Overall, these effects induced by glc-oxLDL were greater than those achieved with oxLDL. Thus, glc-oxLDL activated downstream apoptotic signaling. This may influence the evolution of atherogenesis and vascular complications in diabetes.

AB - Apoptosis of arterial cells induced by oxidized low-density lipoprotein (oxLDL) is thought to contribute to the progression of vascular dysfunction and atherogenesis. It is well established that diabetes mellitus is accompanied by both glycosylation and oxidation of LDL (glc-oxLDL), but the biological effects of these modified lipoproteins are poorly understood. We demonstrate here for the first time that glc-oxLDL increases TUNEL positivity and caspase-3 activation (by Western blot and immunocytochemistry) of human coronary smooth muscle cells. Overall, these effects induced by glc-oxLDL were greater than those achieved with oxLDL. Thus, glc-oxLDL activated downstream apoptotic signaling. This may influence the evolution of atherogenesis and vascular complications in diabetes.

KW - Atherosclerosis

KW - Caspase

KW - glc-oxLDL

KW - oxLDL

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DO - 10.1196/annals.1299.128

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JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

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Glycoxidation of Low-Density Lipoprotein Increases TUNEL Positivity and CPP32 Activation in Human Coronary Cells

Abstract

Keywords

ASJC Scopus subject areas

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