Glycosylation of proteinase 3 (PR3) is not required for its reactivity with antineutrophil cytoplasmic antibodies (ANCA) in Wegener's granulomatosis

Javier D. Finkielman, Peter A. Merkel, Darrell Schroeder, Gary S. Hoffman, Robert Spiera, E. William St. Clair, John C. Davis, W. Joseph McCune, Andrea Lears, Steven R. Ytterberg, Amber M. Hummel, Margaret A. Viss, Tobias Peikert, John H. Stone, Ulrich Specks

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objective. The glycosylation status of autoantigens appears to be crucial for the pathogenesis of some autoimmune diseases, since carbohydrates play a crucial role in the distinction of self from non-self. Proteinase 3 (PR3), the main target antigen for anti-neutrophil cytoplasmic antibodies (ANCA) in patients with Wegener's granulomatosis (WG), contains two Asn-linked glycosylation sites. The present study explores the influence of the glycosylation status of PR3 on the PR3 recognition by ANCA in a well characterized population of patients with WG. Methods. Forty-four patients with WG (459 serum samples) who participated in a multicenter randomized trial, were tested by capture ELISA for ANCA against PR3 and de glycosylated recombinant variants of PR3. Results. The patients were followed for a median of 27 months, and the median number of serum samples per patient was 10. At baseline, the correlation between the levels of ANCA against PR3 and against all the deglycosylated recombinant variants of PR3 were greater than 0.94 (p<0.001 for all the comparisons). Longitudinal analyses comparing the levels of ANCA against PR3 versus all the deglycosylated recombinant variants of PR3, using linear mixed models, showed no significant statistical differences (ρ≥0.90 in all cases). Conclusions. The glycosylation status of PR3 has no impact on its recognition by ANCA in WG.

Original languageEnglish (US)
Pages (from-to)S45-S52
JournalClinical and experimental rheumatology
Volume27
Issue number1 SUPPL. 52
StatePublished - 2009

Keywords

  • ANCA
  • Biological markers
  • Glycosylation
  • Proteinase 3
  • Wegener's granulomatosis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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    Finkielman, J. D., Merkel, P. A., Schroeder, D., Hoffman, G. S., Spiera, R., St. Clair, E. W., Davis, J. C., McCune, W. J., Lears, A., Ytterberg, S. R., Hummel, A. M., Viss, M. A., Peikert, T., Stone, J. H., & Specks, U. (2009). Glycosylation of proteinase 3 (PR3) is not required for its reactivity with antineutrophil cytoplasmic antibodies (ANCA) in Wegener's granulomatosis. Clinical and experimental rheumatology, 27(1 SUPPL. 52), S45-S52.