Glycoprotein hormones and their common A-subunit stimulate prolactin production by explant cultures of human leiomyoma and myometrium

Elizabeth A. Stewart, Mitchell S. Rein, Andrew J. Friedman, Lynn Zuchowski, Romana A. Nowak

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

OBJECTIVE: The purpose of this study was to examine the effect of the gonadotropins luteinizing hormone, follicle-stimulating hormone, and human chorionic gonadotropin on prolactin production by human leiomyomas and myometrium. A secondary goal was to explore the effect of thyroid-stimulating hormone and the A-subunit common to all these glycoproteins in the same system. STUDY DESIGN: Explant cultures were established, and harvested medium was assayed for total protein and prolactin. Dose-response studies were performed with follicle-stimulating hormone, luteinizing hormone, and human chorionic gonadotropin. A second set of studies was conducted with a single dose of human chorionic gonadotropin, thyroid-stimulating hormone, and A-subunit. RESULTS: Gonadotropins, thyroid-stimulating hormone, and A-subunit all stimulated prolactin production in both leiomyoma and myometrium. Prolactin production was significantly higher in leiomyoma than in myometrium. A positive effect of time, dose, and gonadotropin treatment on prolactin production was seen in each tissue. There was no treatment effect on total protein secretion. CONCLUSIONS: All four glycoprotein hormones and their common A-subunit stimulate prolactin production. This appears to be a specific effect. (AM J OBSTET GYNECOL 1994;170:677-83.)

Original languageEnglish (US)
Pages (from-to)677-683
Number of pages7
JournalAmerican journal of obstetrics and gynecology
Volume170
Issue number2
DOIs
StatePublished - Jan 1 1994

Keywords

  • A-Subunit glycoprotein hormones
  • leiomyomas
  • myometrium
  • pituitary gonadotropins
  • prolactin

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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