Glycoprotein hormones and their common α-subunit stimulate prolactin production by explant cultures of human leiomyoma and myometrium

Elizabeth A Stewart, M. S. Rein, A. J. Friedman, L. Zuchowski, R. A. Nowak

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: The purpose of this study was to examine the effect of the gonadotropins luteinizing hormone, follicle-stimulating hormone, and human chorionic gonadotropin on prolactin production by human leiomyomas and myometrium. A secondary goal was to explore the effect of thyroid-stimulating hormone and the α-subunit common to all these glycoproteins in the same system. Study design: Explant cultures were established, and harvested medium was assayed for total protein and prolactin. Dose-response studies were performed with follicle-stimulating hormone, luteinizing hormone, and human chorionic gonadotropin. A second set of studies was conducted with a single dose of human chorionic gonadotropin, thyroid-stimulating hormone, and α-subunit. Results: Gonadotropins, thyroid-stimulating hormone, and α-subunit all stimulated prolactin production in both leiomyoma and myometrium. Prolactin production was significantly higher in leiomyoma than in myometrium. A positive effect of time, dose, and gonadotropin treatment on prolactin production was seen in each tissue. There was no treatment effect on total protein secretion. Conclusions: All four glycoprotein hormones and their common α-subunit stimulate prolactin production. This appears to be a specific effect.

Original languageEnglish (US)
Pages (from-to)677-683
Number of pages7
JournalAmerican Journal of Obstetrics and Gynecology
Volume170
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Myometrium
Leiomyoma
Prolactin
Glycoproteins
Hormones
Thyrotropin
Chorionic Gonadotropin
Gonadotropins
Follicle Stimulating Hormone
Luteinizing Hormone
Proteins

Keywords

  • α-subunit glycoprotein hormones
  • leicomyomas
  • myometrium
  • pituitary gonadotropins
  • prolactin

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Glycoprotein hormones and their common α-subunit stimulate prolactin production by explant cultures of human leiomyoma and myometrium. / Stewart, Elizabeth A; Rein, M. S.; Friedman, A. J.; Zuchowski, L.; Nowak, R. A.

In: American Journal of Obstetrics and Gynecology, Vol. 170, No. 2, 1994, p. 677-683.

Research output: Contribution to journalArticle

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AU - Zuchowski, L.

AU - Nowak, R. A.

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