Glycolipid anchorage of plasmodium falciparum surface antigens

C. Braun Breton, T. L. Rosenberry, L. H. Pereira da Silva

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Human red blood cells (RBC) were infected with the malarial parasite Plasmodium falciparum, the anchoring of schizont proteins to RBC membranes by glycoinositol phospholipids was demonstrated by three criteria: (1) metabolic incorporation of 3H-ethanolamine and 3H-myristate into the protein; (2) release of 35S-methionine-labelled protein into the supernatant after incubation with phosphatidylinositol-specific phospholipase C; and (3) the exposure of a glycoinositol phosphate epitope on the methionine-labelled protein following phospholipase C cleavage. Labelled proteins were analysed by immunoprecipitation, polyacrylamide gel electrophoresis in sodium dodecylsulphate and gel fluorography. Several candidate proteins were observed when each criteria was investigated. Among these, 3 proteins which met all three criteria were identified by immunoprecipitation with monospecific sera or monoclonal antibodies. These included 3 possible vaccine candidates, the p190 major surface antigen, the p76 serine protease and the p71 protein which is thought to be a member of the family of heat-shock Hsp70 proteins.

Original languageEnglish (US)
Pages (from-to)743-755
Number of pages13
JournalResearch in Immunology
Volume141
Issue number7
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Glycolipids
Plasmodium falciparum
Surface Antigens
Proteins
Immunoprecipitation
Methionine
Photofluorography
Erythrocytes
Schizonts
Phosphoinositide Phospholipase C
Ethanolamine
Myristic Acid
Serine Proteases
Type C Phospholipases
Heat-Shock Proteins
Epitopes
Polyacrylamide Gel Electrophoresis
Phospholipids
Parasites
Vaccines

Keywords

  • Glycolipid
  • Malaria
  • Phospholipids
  • Plasmodium falciparum
  • Proteins
  • RBC anchoring
  • Surface antigen
  • Vaccination

ASJC Scopus subject areas

  • Immunology

Cite this

Breton, C. B., Rosenberry, T. L., & Pereira da Silva, L. H. (1990). Glycolipid anchorage of plasmodium falciparum surface antigens. Research in Immunology, 141(7), 743-755. https://doi.org/10.1016/0923-2494(90)90005-J

Glycolipid anchorage of plasmodium falciparum surface antigens. / Breton, C. Braun; Rosenberry, T. L.; Pereira da Silva, L. H.

In: Research in Immunology, Vol. 141, No. 7, 1990, p. 743-755.

Research output: Contribution to journalArticle

Breton, CB, Rosenberry, TL & Pereira da Silva, LH 1990, 'Glycolipid anchorage of plasmodium falciparum surface antigens', Research in Immunology, vol. 141, no. 7, pp. 743-755. https://doi.org/10.1016/0923-2494(90)90005-J
Breton, C. Braun ; Rosenberry, T. L. ; Pereira da Silva, L. H. / Glycolipid anchorage of plasmodium falciparum surface antigens. In: Research in Immunology. 1990 ; Vol. 141, No. 7. pp. 743-755.
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