Glycerol and nonesterified fatty acid metabolism in human muscle and adipose tissue in vivo

Simon W. Coppack, Mai Persson, Robert L. Judd, John M. Miles

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

To determine the relationship between glycerol and nonesterified fatty acid (NEFA) release from adipose tissue, and to test whether forearm muscle and abdominal adipose tissue are capable of extracting these two lipolytic products from the circulation, 13 male subjects were studied after an overnight fast during combined infusion of radiolabeled palmitate and glycerol. Blood samples were taken from a radial artery, a deep forearm vein, and a superficial abdominal vein before and during a 2-h infusion of glucose at ~7 mg · kg-1 · min-1. The ratio of the appearance rates of total NEFA to glycerol was ~3/1 during the baseline period but decreased to 1.3/1 during glucose infusion. There was significant extraction of both glycerol and NEFA by forearm muscle. In contrast, there was no apparent uptake of glycerol by adipose tissue. Adipose tissue NEFA uptake was undetectable during the baseline period but became significant during glucose infusion. These data indicate that there is very little to no in situ reesterification of NEFA in adipose tissue after an overnight fast. During glucose infusion, there was apparently a relative increase in the fraction of glycerol derived from the action of lipoprotein lipase and an increase in reesterification in situ.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume276
Issue number2 39-2
StatePublished - Feb 1999

Fingerprint

Nonesterified Fatty Acids
Metabolism
Glycerol
Muscle
Adipose Tissue
Tissue
Muscles
Forearm
Glucose
Veins
Abdominal Fat
Radial Artery
Lipoprotein Lipase
Palmitates
Blood

Keywords

  • Glucose
  • Insulin
  • Lipolysis
  • Tracer

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Physiology (medical)

Cite this

Glycerol and nonesterified fatty acid metabolism in human muscle and adipose tissue in vivo. / Coppack, Simon W.; Persson, Mai; Judd, Robert L.; Miles, John M.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 276, No. 2 39-2, 02.1999.

Research output: Contribution to journalArticle

Coppack, Simon W. ; Persson, Mai ; Judd, Robert L. ; Miles, John M. / Glycerol and nonesterified fatty acid metabolism in human muscle and adipose tissue in vivo. In: American Journal of Physiology - Endocrinology and Metabolism. 1999 ; Vol. 276, No. 2 39-2.
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