Glutathione reverses endothelial dysfunction and improves nitric oxide bioavailability

Abhiram Prasad, Neil P. Andrews, Feroz A. Padder, Mohsin Husain, Arshed A. Quyyumi

Research output: Contribution to journalArticle

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Abstract

OBJECTIVES: We investigated whether glutathione (GSH), a reduced thiol that modulates redox state and forms adducts of nitric oxide (NO), improves endothelium-dependent vasomotion and NO activity in atherosderosis. BACKGROUND: Endothelial dysfunction and reduced NO activity are associated with atherosclerosis and its clinical manifestations such as unstable angina. METHODS: In the femoral circulation of 17 patients with atherosclerosis or its risk factors, endothelium-dependent vasodilation with acetylcholine (ACH), and endothelium-independent vasodilation with nitroglycerin and sodium nitroprusside were studied before and after GSH. In 10 patients, femoral vein plasma cyclic guanylate monophosphate (cGMP) levels were measured during an infusion of ACH before and after GSH. Femoral artery flow velocity was measured using a Doppler flow wire and the resistance index (FVRI) calculated as mean arterial pressure / flow velocity. RESULTS: Glutathione strongly potentiated ACH-mediated vasodilation; at the two doses, FVRI decreased by 47% and 56% before, and by 61% and 67% after GSH (p = 0.003). Glutathione also elevated cGMP levels in the femoral vein during ACH infusion from 17.6 ± 3 to 23.3 ± 3 pmol/ml (p = 0.006). Augmentation of ACH responses was only observed in patients with depressed endothelial function. Glutathione did not influence endothelium-independent vasodilation with either NO donor. CONCLUSIONS: Thiol supplementation with GSH selectively improves human endothelial dysfunction by enhancing NO activity.

Original languageEnglish (US)
Pages (from-to)507-514
Number of pages8
JournalJournal of the American College of Cardiology
Volume34
Issue number2
DOIs
StatePublished - Aug 1999
Externally publishedYes

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Biological Availability
Acetylcholine
Glutathione
Nitric Oxide
Vasodilation
Endothelium
Femoral Vein
Sulfhydryl Compounds
Atherosclerosis
Nitric Oxide Donors
Unstable Angina
Nitroglycerin
Nitroprusside
Femoral Artery
Thigh
Oxidation-Reduction
Arterial Pressure

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Glutathione reverses endothelial dysfunction and improves nitric oxide bioavailability. / Prasad, Abhiram; Andrews, Neil P.; Padder, Feroz A.; Husain, Mohsin; Quyyumi, Arshed A.

In: Journal of the American College of Cardiology, Vol. 34, No. 2, 08.1999, p. 507-514.

Research output: Contribution to journalArticle

Prasad, Abhiram ; Andrews, Neil P. ; Padder, Feroz A. ; Husain, Mohsin ; Quyyumi, Arshed A. / Glutathione reverses endothelial dysfunction and improves nitric oxide bioavailability. In: Journal of the American College of Cardiology. 1999 ; Vol. 34, No. 2. pp. 507-514.
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abstract = "OBJECTIVES: We investigated whether glutathione (GSH), a reduced thiol that modulates redox state and forms adducts of nitric oxide (NO), improves endothelium-dependent vasomotion and NO activity in atherosderosis. BACKGROUND: Endothelial dysfunction and reduced NO activity are associated with atherosclerosis and its clinical manifestations such as unstable angina. METHODS: In the femoral circulation of 17 patients with atherosclerosis or its risk factors, endothelium-dependent vasodilation with acetylcholine (ACH), and endothelium-independent vasodilation with nitroglycerin and sodium nitroprusside were studied before and after GSH. In 10 patients, femoral vein plasma cyclic guanylate monophosphate (cGMP) levels were measured during an infusion of ACH before and after GSH. Femoral artery flow velocity was measured using a Doppler flow wire and the resistance index (FVRI) calculated as mean arterial pressure / flow velocity. RESULTS: Glutathione strongly potentiated ACH-mediated vasodilation; at the two doses, FVRI decreased by 47{\%} and 56{\%} before, and by 61{\%} and 67{\%} after GSH (p = 0.003). Glutathione also elevated cGMP levels in the femoral vein during ACH infusion from 17.6 ± 3 to 23.3 ± 3 pmol/ml (p = 0.006). Augmentation of ACH responses was only observed in patients with depressed endothelial function. Glutathione did not influence endothelium-independent vasodilation with either NO donor. CONCLUSIONS: Thiol supplementation with GSH selectively improves human endothelial dysfunction by enhancing NO activity.",
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