TY - JOUR
T1 - Glutathione peroxidase activity in rat lens and other tissues in relation to dietary selenium intake
AU - Lawrence, Richard A.
AU - Sunde, Roger A.
AU - Schwartz, Gary L.
AU - Hoekstra, William G.
N1 - Funding Information:
This researchw as supportedb y the Collegeo f Agricultural and Life Sciences,U niversity of Wisconsin, Madison, by United States Public Health Service Program Grant no. AM-14881 and by NIH training grant GM 00236B CH (to R. A. L.).
PY - 1974/6
Y1 - 1974/6
N2 - Glutathione peroxidase (E.C. 1.11.1.9: glutathione: H2O2 oxidoreductase) activity and selenium concentration were measured in lenses of female rats and their offspring after long-term feeding of either a selenium-supplemented ( 0·1 parts 106) or selenium-deficient (< 0·02 parts 106) diet. Long-term selenium deficiency decreased lens glutathione peroxidase activity in parent rats and their offspring to 15 and 14% respectively of supplemented controls. For comparison to lens, glutathione peroxidase was also measured in liver, heart, lung, erythrocytes, kidney, adrenal, testis, and brain of the offspring. Selenium deficiency caused the enzyme to decrease most dramatically in liver (to 0) and least in brain (to 62% of selenium supplemented controls). Although glutathione peroxidase in lens was lower than that in the other organs assayed, it was among the organs more sensitive to depletion caused by selenium deficiency. A short-term selenium deficiency of 8 weeks in newborn lambs had no effect on lens glutathione peroxidase, but the enzyme in organs such as liver was dramatically decreased. Therefore, an extensive period of selenium deficiency appears necessary to affect lens glutathione peroxidase activity, which probably relates to the relatively slow turnover and slow growth of the lens. The possible role of the seleno-enzyme, glutathione peroxidase, in the prevention of cataracts and the relationship of selenium to vitamin E and sulfur-containing amino acids in this regard are discussed.
AB - Glutathione peroxidase (E.C. 1.11.1.9: glutathione: H2O2 oxidoreductase) activity and selenium concentration were measured in lenses of female rats and their offspring after long-term feeding of either a selenium-supplemented ( 0·1 parts 106) or selenium-deficient (< 0·02 parts 106) diet. Long-term selenium deficiency decreased lens glutathione peroxidase activity in parent rats and their offspring to 15 and 14% respectively of supplemented controls. For comparison to lens, glutathione peroxidase was also measured in liver, heart, lung, erythrocytes, kidney, adrenal, testis, and brain of the offspring. Selenium deficiency caused the enzyme to decrease most dramatically in liver (to 0) and least in brain (to 62% of selenium supplemented controls). Although glutathione peroxidase in lens was lower than that in the other organs assayed, it was among the organs more sensitive to depletion caused by selenium deficiency. A short-term selenium deficiency of 8 weeks in newborn lambs had no effect on lens glutathione peroxidase, but the enzyme in organs such as liver was dramatically decreased. Therefore, an extensive period of selenium deficiency appears necessary to affect lens glutathione peroxidase activity, which probably relates to the relatively slow turnover and slow growth of the lens. The possible role of the seleno-enzyme, glutathione peroxidase, in the prevention of cataracts and the relationship of selenium to vitamin E and sulfur-containing amino acids in this regard are discussed.
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U2 - 10.1016/0014-4835(74)90062-1
DO - 10.1016/0014-4835(74)90062-1
M3 - Article
C2 - 4852169
AN - SCOPUS:0016150712
SN - 0014-4835
VL - 18
SP - 563
EP - 569
JO - Experimental Eye Research
JF - Experimental Eye Research
IS - 6
ER -