Glutamate-induced protease-mediated loss of plasma membrane Ca2+ pump activity in rat hippocampal neurons

William J. Pottorf, Tanner M. Johanns, Stephen M. Derrington, Emanuel E. Strehler, Agnes Enyedi, Stanley A. Thayer

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Abstract

Ca2+ dysregulation is a hallmark of excitotoxicity, a process that underlies multiple neurodegenerative disorders. The plasma membrane Ca 2+ ATPase (PMCA) plays a major role in clearing Ca2+ from the neuronal cytoplasm. Here, we show that the rate of PMCA-mediated Ca 2+ efflux from rat hippocampal neurons decreased following treatment with an excitotoxic concentration of glutamate. PMCA-mediated Ca2+ extrusion following a brief train of action potentials exhibited an exponential decay with a mean time constant (τ) of 8.8 ± 0.2 s. Four hours following the start of a 30 min treatment with 200 μm glutamate, a second population of cells emerged with slowed recovery kinetics (τ = 16.5 ± 0.3 s). Confocal imaging of cells expressing an enhanced green fluorescent protein (EGFP)-PMCA4b fusion protein revealed that glutamate treatment internalized EGFP and that cells with reduced plasma membrane fluorescence had impaired Ca2+ clearance. Treatment with inhibitors of the Ca 2+-activated protease calpain protected PMCA function and prevented EGFP-PMCA internalization. PMCA internalization was triggered by activation of NMDA receptors and was less pronounced for a non-toxic concentration of glutamate relative to one that produces excitotoxicity. PMCA isoform 2 also internalized following exposure to glutamate, although the Na+/K + ATPase did not. These data suggest that glutamate exposure initiated protease-mediated internalization of PMCAs with a corresponding loss of function that may contribute to the Ca2+ dysregulation that accompanies excitotoxicity.

Original languageEnglish (US)
Pages (from-to)1646-1656
Number of pages11
JournalJournal of Neurochemistry
Volume98
Issue number5
DOIs
StatePublished - Sep 2006

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Cell membranes
Neurons
Adenosine Triphosphatases
Rats
Glutamic Acid
Peptide Hydrolases
Cell Membrane
Pumps
Cells
Calpain
Calcium-Transporting ATPases
N-Methyl-D-Aspartate Receptors
Neurodegenerative Diseases
Action Potentials
Extrusion
Protein Isoforms
Cytoplasm
Fusion reactions
Fluorescence
Chemical activation

Keywords

  • Calpain
  • Caspase
  • Excitotoxicity
  • Glutamate
  • Plasma membrane calcium ATPase
  • Plasma membrane calcium pump

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Pottorf, W. J., Johanns, T. M., Derrington, S. M., Strehler, E. E., Enyedi, A., & Thayer, S. A. (2006). Glutamate-induced protease-mediated loss of plasma membrane Ca2+ pump activity in rat hippocampal neurons. Journal of Neurochemistry, 98(5), 1646-1656. https://doi.org/10.1111/j.1471-4159.2006.04063.x

Glutamate-induced protease-mediated loss of plasma membrane Ca2+ pump activity in rat hippocampal neurons. / Pottorf, William J.; Johanns, Tanner M.; Derrington, Stephen M.; Strehler, Emanuel E.; Enyedi, Agnes; Thayer, Stanley A.

In: Journal of Neurochemistry, Vol. 98, No. 5, 09.2006, p. 1646-1656.

Research output: Contribution to journalArticle

Pottorf, WJ, Johanns, TM, Derrington, SM, Strehler, EE, Enyedi, A & Thayer, SA 2006, 'Glutamate-induced protease-mediated loss of plasma membrane Ca2+ pump activity in rat hippocampal neurons', Journal of Neurochemistry, vol. 98, no. 5, pp. 1646-1656. https://doi.org/10.1111/j.1471-4159.2006.04063.x
Pottorf, William J. ; Johanns, Tanner M. ; Derrington, Stephen M. ; Strehler, Emanuel E. ; Enyedi, Agnes ; Thayer, Stanley A. / Glutamate-induced protease-mediated loss of plasma membrane Ca2+ pump activity in rat hippocampal neurons. In: Journal of Neurochemistry. 2006 ; Vol. 98, No. 5. pp. 1646-1656.
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