Glucose, lactate, and pyruvate biosensor arrays based on redox polymer/oxidoreductase nanocomposite thin-films deposited on photolithographically patterned gold microelectrodes

Alexander Revzin, Kaushik Sirkar, Aleksandr Simonian, Michael V. Pishko

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Glucose, lactate, and pyruvate sensor arrays were fabricated by depositing electrostatically complexed monolayers on lithographically patterned, individually addressable, gold microelectrodes. Standard photolithographic techniques combined with metal deposition were used to fabricate gold arrays on both SiO2/Si and flexible Mylar substrates. These gold arrays were then functionalized with a negative surface charge through chemisorption of 11-mercaptoundecanoic acid (MUA) followed by the electrostatic assembly of a nanocomposite thin-film of a cationic osmium redox polymer and anionic oxidoreductases, either glucose oxidase, lactate oxidase, or pyruvate oxidase. When tested electrochemically, glucose, lactate and pyruvate sensors exhibited analyte sensitivities of 0.26, 0.24 and 0.133 μA/(cm2 mM) respectively. Responses to analytes proved to be linear in the physiologically relevant concentration ranges for glucose (0-20 mM), lactate (0-10 mM), and pyruvate (0-2 mM). Standard deviations between individual electrodes of ∼18% (glucose) and 20% (lactate) were determined for the enzyme electrode arrays with five array members. Furthermore, the potential problem of sensor cross-talk was investigated by subsequently testing one array member and then array members adjacent to that sensor. The response from a pair of electrodes was approximately twice than that of a single electrode, demonstrating that the individual sensors are free of cross-talk.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalSensors and Actuators, B: Chemical
Volume81
Issue number2-3
DOIs
StatePublished - Jan 5 2002
Externally publishedYes

Fingerprint

pyruvates
lactates
Nanocomposite films
Microelectrodes
Pyruvic Acid
bioinstrumentation
Biosensors
glucose
Gold
Glucose
Lactic Acid
nanocomposites
Oxidoreductases
Polymers
gold
Thin films
lactate 2-monooxygenase
Sensors
polymers
thin films

Keywords

  • Electrode arrays
  • Electrostatic assembly
  • Enzyme
  • Redox polymer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Glucose, lactate, and pyruvate biosensor arrays based on redox polymer/oxidoreductase nanocomposite thin-films deposited on photolithographically patterned gold microelectrodes. / Revzin, Alexander; Sirkar, Kaushik; Simonian, Aleksandr; Pishko, Michael V.

In: Sensors and Actuators, B: Chemical, Vol. 81, No. 2-3, 05.01.2002, p. 359-368.

Research output: Contribution to journalArticle

@article{ab35cee31c5c45d2bb0e794b81218be4,
title = "Glucose, lactate, and pyruvate biosensor arrays based on redox polymer/oxidoreductase nanocomposite thin-films deposited on photolithographically patterned gold microelectrodes",
abstract = "Glucose, lactate, and pyruvate sensor arrays were fabricated by depositing electrostatically complexed monolayers on lithographically patterned, individually addressable, gold microelectrodes. Standard photolithographic techniques combined with metal deposition were used to fabricate gold arrays on both SiO2/Si and flexible Mylar substrates. These gold arrays were then functionalized with a negative surface charge through chemisorption of 11-mercaptoundecanoic acid (MUA) followed by the electrostatic assembly of a nanocomposite thin-film of a cationic osmium redox polymer and anionic oxidoreductases, either glucose oxidase, lactate oxidase, or pyruvate oxidase. When tested electrochemically, glucose, lactate and pyruvate sensors exhibited analyte sensitivities of 0.26, 0.24 and 0.133 μA/(cm2 mM) respectively. Responses to analytes proved to be linear in the physiologically relevant concentration ranges for glucose (0-20 mM), lactate (0-10 mM), and pyruvate (0-2 mM). Standard deviations between individual electrodes of ∼18{\%} (glucose) and 20{\%} (lactate) were determined for the enzyme electrode arrays with five array members. Furthermore, the potential problem of sensor cross-talk was investigated by subsequently testing one array member and then array members adjacent to that sensor. The response from a pair of electrodes was approximately twice than that of a single electrode, demonstrating that the individual sensors are free of cross-talk.",
keywords = "Electrode arrays, Electrostatic assembly, Enzyme, Redox polymer",
author = "Alexander Revzin and Kaushik Sirkar and Aleksandr Simonian and Pishko, {Michael V.}",
year = "2002",
month = "1",
day = "5",
doi = "10.1016/S0925-4005(01)00982-0",
language = "English (US)",
volume = "81",
pages = "359--368",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Glucose, lactate, and pyruvate biosensor arrays based on redox polymer/oxidoreductase nanocomposite thin-films deposited on photolithographically patterned gold microelectrodes

AU - Revzin, Alexander

AU - Sirkar, Kaushik

AU - Simonian, Aleksandr

AU - Pishko, Michael V.

PY - 2002/1/5

Y1 - 2002/1/5

N2 - Glucose, lactate, and pyruvate sensor arrays were fabricated by depositing electrostatically complexed monolayers on lithographically patterned, individually addressable, gold microelectrodes. Standard photolithographic techniques combined with metal deposition were used to fabricate gold arrays on both SiO2/Si and flexible Mylar substrates. These gold arrays were then functionalized with a negative surface charge through chemisorption of 11-mercaptoundecanoic acid (MUA) followed by the electrostatic assembly of a nanocomposite thin-film of a cationic osmium redox polymer and anionic oxidoreductases, either glucose oxidase, lactate oxidase, or pyruvate oxidase. When tested electrochemically, glucose, lactate and pyruvate sensors exhibited analyte sensitivities of 0.26, 0.24 and 0.133 μA/(cm2 mM) respectively. Responses to analytes proved to be linear in the physiologically relevant concentration ranges for glucose (0-20 mM), lactate (0-10 mM), and pyruvate (0-2 mM). Standard deviations between individual electrodes of ∼18% (glucose) and 20% (lactate) were determined for the enzyme electrode arrays with five array members. Furthermore, the potential problem of sensor cross-talk was investigated by subsequently testing one array member and then array members adjacent to that sensor. The response from a pair of electrodes was approximately twice than that of a single electrode, demonstrating that the individual sensors are free of cross-talk.

AB - Glucose, lactate, and pyruvate sensor arrays were fabricated by depositing electrostatically complexed monolayers on lithographically patterned, individually addressable, gold microelectrodes. Standard photolithographic techniques combined with metal deposition were used to fabricate gold arrays on both SiO2/Si and flexible Mylar substrates. These gold arrays were then functionalized with a negative surface charge through chemisorption of 11-mercaptoundecanoic acid (MUA) followed by the electrostatic assembly of a nanocomposite thin-film of a cationic osmium redox polymer and anionic oxidoreductases, either glucose oxidase, lactate oxidase, or pyruvate oxidase. When tested electrochemically, glucose, lactate and pyruvate sensors exhibited analyte sensitivities of 0.26, 0.24 and 0.133 μA/(cm2 mM) respectively. Responses to analytes proved to be linear in the physiologically relevant concentration ranges for glucose (0-20 mM), lactate (0-10 mM), and pyruvate (0-2 mM). Standard deviations between individual electrodes of ∼18% (glucose) and 20% (lactate) were determined for the enzyme electrode arrays with five array members. Furthermore, the potential problem of sensor cross-talk was investigated by subsequently testing one array member and then array members adjacent to that sensor. The response from a pair of electrodes was approximately twice than that of a single electrode, demonstrating that the individual sensors are free of cross-talk.

KW - Electrode arrays

KW - Electrostatic assembly

KW - Enzyme

KW - Redox polymer

UR - http://www.scopus.com/inward/record.url?scp=0037021793&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037021793&partnerID=8YFLogxK

U2 - 10.1016/S0925-4005(01)00982-0

DO - 10.1016/S0925-4005(01)00982-0

M3 - Article

VL - 81

SP - 359

EP - 368

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 2-3

ER -