Glucose, lactate, and pyruvate biosensor arrays based on redox polymer/oxidoreductase nanocomposite thin-films deposited on photolithographically patterned gold microelectrodes

Alexander F. Revzin, Kaushik Sirkar, Aleksandr Simonian, Michael V. Pishko

Research output: Contribution to journalArticle

76 Scopus citations


Glucose, lactate, and pyruvate sensor arrays were fabricated by depositing electrostatically complexed monolayers on lithographically patterned, individually addressable, gold microelectrodes. Standard photolithographic techniques combined with metal deposition were used to fabricate gold arrays on both SiO2/Si and flexible Mylar substrates. These gold arrays were then functionalized with a negative surface charge through chemisorption of 11-mercaptoundecanoic acid (MUA) followed by the electrostatic assembly of a nanocomposite thin-film of a cationic osmium redox polymer and anionic oxidoreductases, either glucose oxidase, lactate oxidase, or pyruvate oxidase. When tested electrochemically, glucose, lactate and pyruvate sensors exhibited analyte sensitivities of 0.26, 0.24 and 0.133 μA/(cm2 mM) respectively. Responses to analytes proved to be linear in the physiologically relevant concentration ranges for glucose (0-20 mM), lactate (0-10 mM), and pyruvate (0-2 mM). Standard deviations between individual electrodes of ∼18% (glucose) and 20% (lactate) were determined for the enzyme electrode arrays with five array members. Furthermore, the potential problem of sensor cross-talk was investigated by subsequently testing one array member and then array members adjacent to that sensor. The response from a pair of electrodes was approximately twice than that of a single electrode, demonstrating that the individual sensors are free of cross-talk.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalSensors and Actuators, B: Chemical
Issue number2-3
StatePublished - Jan 5 2002



  • Electrode arrays
  • Electrostatic assembly
  • Enzyme
  • Redox polymer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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