Glucose clamping using the biostator GCIIS

C. A. Verdonk, R. A. Rizza, R. E. Westland, R. L. Nelson, J. E. Gerich, F. J. Service

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Abstract

The Biostator GCIIS was used to clamp circulating glucose at hypoglycemic (42 ± 1 mg/dl), euglycemic (86 ± 2 mg/dl), and hyperglycemic (142 ± 1 mg/dl) levels in normal subjects during a concomitant infusion of insulin (0.1 U/kg/h). Because of limitations in maximal glucose infusion (1 g/min) from the Biostator, a supplementary infusion of glucose was required to accomplish euglycemic and hyperglycemic clamps. The coefficients of variation of blood glucose were 7.06 ± 1.3%, 5.9 ± 0.5%, and 6.1 ± 0.9 for 120 minutes of hypoglycemic, euglycemic, and hyperglycemic clamps, respectively. Despite the occasional interruption of blood flow in the double-lumen catheter and the occasional poor correlation between Biostator and reference glucose method, satisfactory glucose clamps could be maintained for 2 hours.

Original languageEnglish (US)
Pages (from-to)133-135
Number of pages3
JournalHormone and Metabolic Research
Volume12
Issue number4
StatePublished - 1980

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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Verdonk, C. A., Rizza, R. A., Westland, R. E., Nelson, R. L., Gerich, J. E., & Service, F. J. (1980). Glucose clamping using the biostator GCIIS. Hormone and Metabolic Research, 12(4), 133-135.