Glucose-6-phosphate dehydrogenase activity in term and near-term, male African American neonates

Michael Kaplan, James Hoyer, Marguerite Herschel, Cathy Hammerman, David K. Stevenson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: We determined values for glucose-6-phosphate dehydrogenase (G-6-PD) activity in African American neonates. Methods: G-6-PD activity was measured on umbilical cord blood from term and near-term healthy, male neonates. Neonates were stratified according to the number of neonates for each numerical unit of G-6-PD activity. Corrected end tidal carbon monoxide (ETCOc), a non-invasive index of hemolysis, was performed on each neonate. At least one predischarge transcutanous bilirubin determination was performed. Results: Five hundred neonates were studied. Two subpopulations were apparent, with no overlap between the subgroups. Mean value for the 64 (12.8%) infants with the lower values (G-6-PD deficient) was 2.7±1.1 U/g Hb, range 0.4-6.6 U/g Hb, while that for the 436 neonates with the higher values (G-6-PD normal) was 21.8±2.9 U/g Hb, range 14.5-33.8 U/g Hb. No significant differences in activity were noted between those neonates <37 weeks gestational age and those >37 weeks. Enzyme activity in the lower range in both groups was not related to the development of hyperbilirubinemia. G-6-PD enzyme activity did not correlate with ETCOc values either for the entire cohort or for the individual subsets. Conclusions: G-6-PD-deficient neonates formed a separate subgroup from those with normal enzyme activity. The data supplied should facilitate interpretation of G-6-PD test results.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalClinica Chimica Acta
Volume355
Issue number1-2
DOIs
StatePublished - Jan 1 2005

Fingerprint

Glucosephosphate Dehydrogenase
African Americans
Newborn Infant
Enzyme activity
Enzymes
Hyperbilirubinemia
Carbon Monoxide
Bilirubin
Hemolysis
Fetal Blood
Blood

Keywords

  • African American
  • End tidal carbon monoxide
  • Enzyme activity
  • Glucose-6-phosphate dehydrogenase
  • Hemolysis
  • Neonatal hyperbilirubinemia

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Glucose-6-phosphate dehydrogenase activity in term and near-term, male African American neonates. / Kaplan, Michael; Hoyer, James; Herschel, Marguerite; Hammerman, Cathy; Stevenson, David K.

In: Clinica Chimica Acta, Vol. 355, No. 1-2, 01.01.2005, p. 113-117.

Research output: Contribution to journalArticle

Kaplan, Michael ; Hoyer, James ; Herschel, Marguerite ; Hammerman, Cathy ; Stevenson, David K. / Glucose-6-phosphate dehydrogenase activity in term and near-term, male African American neonates. In: Clinica Chimica Acta. 2005 ; Vol. 355, No. 1-2. pp. 113-117.
@article{726eab35492645d58fcbbf1eab503a59,
title = "Glucose-6-phosphate dehydrogenase activity in term and near-term, male African American neonates",
abstract = "Background: We determined values for glucose-6-phosphate dehydrogenase (G-6-PD) activity in African American neonates. Methods: G-6-PD activity was measured on umbilical cord blood from term and near-term healthy, male neonates. Neonates were stratified according to the number of neonates for each numerical unit of G-6-PD activity. Corrected end tidal carbon monoxide (ETCOc), a non-invasive index of hemolysis, was performed on each neonate. At least one predischarge transcutanous bilirubin determination was performed. Results: Five hundred neonates were studied. Two subpopulations were apparent, with no overlap between the subgroups. Mean value for the 64 (12.8{\%}) infants with the lower values (G-6-PD deficient) was 2.7±1.1 U/g Hb, range 0.4-6.6 U/g Hb, while that for the 436 neonates with the higher values (G-6-PD normal) was 21.8±2.9 U/g Hb, range 14.5-33.8 U/g Hb. No significant differences in activity were noted between those neonates <37 weeks gestational age and those >37 weeks. Enzyme activity in the lower range in both groups was not related to the development of hyperbilirubinemia. G-6-PD enzyme activity did not correlate with ETCOc values either for the entire cohort or for the individual subsets. Conclusions: G-6-PD-deficient neonates formed a separate subgroup from those with normal enzyme activity. The data supplied should facilitate interpretation of G-6-PD test results.",
keywords = "African American, End tidal carbon monoxide, Enzyme activity, Glucose-6-phosphate dehydrogenase, Hemolysis, Neonatal hyperbilirubinemia",
author = "Michael Kaplan and James Hoyer and Marguerite Herschel and Cathy Hammerman and Stevenson, {David K.}",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/j.cccn.2004.12.008",
language = "English (US)",
volume = "355",
pages = "113--117",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Glucose-6-phosphate dehydrogenase activity in term and near-term, male African American neonates

AU - Kaplan, Michael

AU - Hoyer, James

AU - Herschel, Marguerite

AU - Hammerman, Cathy

AU - Stevenson, David K.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Background: We determined values for glucose-6-phosphate dehydrogenase (G-6-PD) activity in African American neonates. Methods: G-6-PD activity was measured on umbilical cord blood from term and near-term healthy, male neonates. Neonates were stratified according to the number of neonates for each numerical unit of G-6-PD activity. Corrected end tidal carbon monoxide (ETCOc), a non-invasive index of hemolysis, was performed on each neonate. At least one predischarge transcutanous bilirubin determination was performed. Results: Five hundred neonates were studied. Two subpopulations were apparent, with no overlap between the subgroups. Mean value for the 64 (12.8%) infants with the lower values (G-6-PD deficient) was 2.7±1.1 U/g Hb, range 0.4-6.6 U/g Hb, while that for the 436 neonates with the higher values (G-6-PD normal) was 21.8±2.9 U/g Hb, range 14.5-33.8 U/g Hb. No significant differences in activity were noted between those neonates <37 weeks gestational age and those >37 weeks. Enzyme activity in the lower range in both groups was not related to the development of hyperbilirubinemia. G-6-PD enzyme activity did not correlate with ETCOc values either for the entire cohort or for the individual subsets. Conclusions: G-6-PD-deficient neonates formed a separate subgroup from those with normal enzyme activity. The data supplied should facilitate interpretation of G-6-PD test results.

AB - Background: We determined values for glucose-6-phosphate dehydrogenase (G-6-PD) activity in African American neonates. Methods: G-6-PD activity was measured on umbilical cord blood from term and near-term healthy, male neonates. Neonates were stratified according to the number of neonates for each numerical unit of G-6-PD activity. Corrected end tidal carbon monoxide (ETCOc), a non-invasive index of hemolysis, was performed on each neonate. At least one predischarge transcutanous bilirubin determination was performed. Results: Five hundred neonates were studied. Two subpopulations were apparent, with no overlap between the subgroups. Mean value for the 64 (12.8%) infants with the lower values (G-6-PD deficient) was 2.7±1.1 U/g Hb, range 0.4-6.6 U/g Hb, while that for the 436 neonates with the higher values (G-6-PD normal) was 21.8±2.9 U/g Hb, range 14.5-33.8 U/g Hb. No significant differences in activity were noted between those neonates <37 weeks gestational age and those >37 weeks. Enzyme activity in the lower range in both groups was not related to the development of hyperbilirubinemia. G-6-PD enzyme activity did not correlate with ETCOc values either for the entire cohort or for the individual subsets. Conclusions: G-6-PD-deficient neonates formed a separate subgroup from those with normal enzyme activity. The data supplied should facilitate interpretation of G-6-PD test results.

KW - African American

KW - End tidal carbon monoxide

KW - Enzyme activity

KW - Glucose-6-phosphate dehydrogenase

KW - Hemolysis

KW - Neonatal hyperbilirubinemia

UR - http://www.scopus.com/inward/record.url?scp=17044411329&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17044411329&partnerID=8YFLogxK

U2 - 10.1016/j.cccn.2004.12.008

DO - 10.1016/j.cccn.2004.12.008

M3 - Article

VL - 355

SP - 113

EP - 117

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1-2

ER -