Glucocorticoids potentiate the prostaglandin E1-mediated cyclic AMP formation by a cultured murine neuroblastoma clone

C. Forray, E. Richelson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The effect of steroid hormones on the prostaglandin E1 (PGE1)-mediated cyclic AMP formation by murine neuroblastoma clone N1E-115 was studied. Dexamethasone at submicromolar concentrations and corticosterone at micromolar concentrations (steroids with glucocorticoid activity) were able to modify the PGE1-mediated response whereas testosterone, progesterone, and estradiol each at 10 μM had no effect. Glucocorticoids added to the culture medium of N1E-115 cells produced an increase in the maximal response to PGE1 only after long-term (≥ 4 h) incubation with the hormone. Inhibitors of protein and RNA synthesis blocked this effect of glucocorticoids. Basal activity of adenylate cyclase in treated cells was twofold higher than that in control cells, and this enzyme seemed to be the primary target for the hormone action, since the activity of 3':5'-cyclic AMP phosphodiesterase and the binding of [3H]PGE1 to its receptors were not altered by glucocorticoid treatment. Our results indicate that glucocorticoids modulate receptor-mediated responses in cells of neural origin through a mechanism that involves induction of protein synthesis.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalJournal of Neurochemistry
Volume45
Issue number1
StatePublished - 1985

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Alprostadil
Neuroblastoma
Cyclic AMP
Glucocorticoids
Clone Cells
Hormones
Steroid hormones
Steroids
Nucleic Acid Synthesis Inhibitors
Protein Synthesis Inhibitors
Glucocorticoid Receptors
Phosphoric Diester Hydrolases
Corticosterone
Adenylyl Cyclases
Dexamethasone
Progesterone
Culture Media
Testosterone
Estradiol
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Glucocorticoids potentiate the prostaglandin E1-mediated cyclic AMP formation by a cultured murine neuroblastoma clone. / Forray, C.; Richelson, E.

In: Journal of Neurochemistry, Vol. 45, No. 1, 1985, p. 79-85.

Research output: Contribution to journalArticle

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