Glucocorticoid inhibition of initiation of transcription of the DNA encoding rRNA (rDNA) in lymphosarcoma P1798 cells

A. H. Cavanaugh, P. K. Gokal, R. P. Lawther, E. A. Thompson

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Cell-free extracts of lymphosarcoma P1798 cell culture lines support faithful initiation upon the cloned mouse DNA encoding rRNA (rDNA) promoter, whereas extracts from cells treated for 16 hr with 0.1 μM dexamethasone cannot. Extracts from both sources transcribe the cloned 5S RNA gene in vitro and mixing experiments further demonstrate that inhibition of transcription of rDNA in vitro is not due to nucleases or inhibitors of transcription present in extracts from glucocorticoid-treated cells. Incubation of extracts from control cells at 45° C for 15 min inactivates RNA polymerase I and abolishes transcription. Activity can be restored by the addition of partially prufied RNA polymerase I from control cells and hormone-treated cells. Moreover, extracts from hormone treated cells can be reconstituted by the addition of a partially purified, heat-stable transcription factor from control cells.

Original languageEnglish (US)
Pages (from-to)718-721
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number3 I
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • General

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