Glucocorticoid binding properties of mouse thymic lymphosarcoma cell populations selected for resistance to the cytolytic effects of glucocorticoids in vivo

A. K. Chan, E Aubrey Thompson

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4 Citations (Scopus)

Abstract

Thirteen independently derived murine thymic lymphosarcoma lines were assayed for various aspects of sensitivity to glucocorticoid-induced cytolysis. All tumor lines were sensitive to cytolysis, as evidenced by profound tumor regression after pharmacologic doses of cortisol. All tumor lines contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 55 and 88% of these presumptive receptor sites underwent nuclear translocation during a 30-minute incubation at 37°C. Dissociation constants (K(d)) for the dexamethasone-receptor complex were between 1.5 and 3.6 nM in all cases. K(d) for the triamcinolone acetonide-receptor complex were determined for a few tumor lines and were between 0.5 and 0.9 nM. Cytolysis-resistant subpopulations were selected by prolonged glucocorticoid treatment of BALB/cπ mice bearing tumors from seven of the lymphosarcoma lines. All seven resistant subpopulations contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 57 and 80% of these presumptive receptor sites underwent nuclear translocation under standard assay conditions. No resistant variants exhibited significantly reduced dexamethasone binding or nuclear translocation properties.

Original languageEnglish (US)
Pages (from-to)579-582
Number of pages4
JournalJournal of the National Cancer Institute
Volume71
Issue number3
StatePublished - 1983
Externally publishedYes

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Non-Hodgkin's Lymphoma
Glucocorticoids
Population
Neoplasms
Dexamethasone
Hydrocortisone
dexamethasone receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Glucocorticoid binding properties of mouse thymic lymphosarcoma cell populations selected for resistance to the cytolytic effects of glucocorticoids in vivo",
abstract = "Thirteen independently derived murine thymic lymphosarcoma lines were assayed for various aspects of sensitivity to glucocorticoid-induced cytolysis. All tumor lines were sensitive to cytolysis, as evidenced by profound tumor regression after pharmacologic doses of cortisol. All tumor lines contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 55 and 88{\%} of these presumptive receptor sites underwent nuclear translocation during a 30-minute incubation at 37°C. Dissociation constants (K(d)) for the dexamethasone-receptor complex were between 1.5 and 3.6 nM in all cases. K(d) for the triamcinolone acetonide-receptor complex were determined for a few tumor lines and were between 0.5 and 0.9 nM. Cytolysis-resistant subpopulations were selected by prolonged glucocorticoid treatment of BALB/cπ mice bearing tumors from seven of the lymphosarcoma lines. All seven resistant subpopulations contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 57 and 80{\%} of these presumptive receptor sites underwent nuclear translocation under standard assay conditions. No resistant variants exhibited significantly reduced dexamethasone binding or nuclear translocation properties.",
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T1 - Glucocorticoid binding properties of mouse thymic lymphosarcoma cell populations selected for resistance to the cytolytic effects of glucocorticoids in vivo

AU - Chan, A. K.

AU - Thompson, E Aubrey

PY - 1983

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N2 - Thirteen independently derived murine thymic lymphosarcoma lines were assayed for various aspects of sensitivity to glucocorticoid-induced cytolysis. All tumor lines were sensitive to cytolysis, as evidenced by profound tumor regression after pharmacologic doses of cortisol. All tumor lines contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 55 and 88% of these presumptive receptor sites underwent nuclear translocation during a 30-minute incubation at 37°C. Dissociation constants (K(d)) for the dexamethasone-receptor complex were between 1.5 and 3.6 nM in all cases. K(d) for the triamcinolone acetonide-receptor complex were determined for a few tumor lines and were between 0.5 and 0.9 nM. Cytolysis-resistant subpopulations were selected by prolonged glucocorticoid treatment of BALB/cπ mice bearing tumors from seven of the lymphosarcoma lines. All seven resistant subpopulations contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 57 and 80% of these presumptive receptor sites underwent nuclear translocation under standard assay conditions. No resistant variants exhibited significantly reduced dexamethasone binding or nuclear translocation properties.

AB - Thirteen independently derived murine thymic lymphosarcoma lines were assayed for various aspects of sensitivity to glucocorticoid-induced cytolysis. All tumor lines were sensitive to cytolysis, as evidenced by profound tumor regression after pharmacologic doses of cortisol. All tumor lines contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 55 and 88% of these presumptive receptor sites underwent nuclear translocation during a 30-minute incubation at 37°C. Dissociation constants (K(d)) for the dexamethasone-receptor complex were between 1.5 and 3.6 nM in all cases. K(d) for the triamcinolone acetonide-receptor complex were determined for a few tumor lines and were between 0.5 and 0.9 nM. Cytolysis-resistant subpopulations were selected by prolonged glucocorticoid treatment of BALB/cπ mice bearing tumors from seven of the lymphosarcoma lines. All seven resistant subpopulations contained about 20,000 high-affinity, dexamethasone binding sites/cell. Between 57 and 80% of these presumptive receptor sites underwent nuclear translocation under standard assay conditions. No resistant variants exhibited significantly reduced dexamethasone binding or nuclear translocation properties.

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