Global cytokine analysis in myeloproliferative disorders

Ching Liang Ho, Terra L. Lasho, Joseph H. Butterfield, Ayalew Tefferi

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Recently developed human cytokine array membranes combine the individual assets of enzyme-linked immunosorbent assay, enhanced chemiluminescence, and the high-throughput of microspot in order to detect multiple plasma cytokines simultaneously. We employed such a system to evaluate the presence and quantity of 79 different cytokines in the plasma of 20 patients with myeloproliferative disorders (MPDs) that were not on active therapy: 10 with primary myelofibrosis (PMF), 5 with polycythemia vera (PV), and 5 with essential thrombocythemia (ET). Compared to healthy controls, patients with PMF, but not those with either PV or ET, displayed significantly higher levels of tissue inhibitor of metalloproteinase (TIMP-1), macrophage inflammatory protein-1β (MIP-1β), and insulin-like growth factor binding factor-2 (IGFBP-2) (p = 0.013, 0.028 and 0.02, respectively). These results constitute an important conformation for the pathogenetic role of these cytokines in PMF.

Original languageEnglish (US)
Pages (from-to)1389-1392
Number of pages4
JournalLeukemia Research
Volume31
Issue number10
DOIs
StatePublished - Oct 1 2007

Keywords

  • Bone marrow stroma
  • Cytokine array
  • Myelofibrosis
  • Pathogenesis

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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