TY - JOUR
T1 - Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile
AU - Kagohara, Luciane Tsukamoto
AU - Schussel, Juliana L.
AU - Subbannayya, Tejaswini
AU - Sahasrabuddhe, Nandini
AU - Lebron, Cynthia
AU - Brait, Mariana
AU - Maldonado, Leonel
AU - Valle, Blanca L.
AU - Pirini, Francesca
AU - Jahuira, Martha
AU - Lopez, Jaime
AU - Letelier, Pablo
AU - Brebi-Mieville, Priscilla
AU - Ili, Carmen
AU - Pandey, Akhilesh
AU - Chatterjee, Aditi
AU - Sidransky, David
AU - Guerrero-Preston, Rafael
N1 - Publisher Copyright:
© 2015 Future Medicine Ltd.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Aim: The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. Material & methods: DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR. Results: Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%. Conclusion: Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.
AB - Aim: The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. Material & methods: DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR. Results: Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%. Conclusion: Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.
KW - gallbladder cancer
KW - global and gene-specific DNA methylation
KW - molecular biomarkers panel
KW - tumor suppressor genes
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UR - http://www.scopus.com/inward/citedby.url?scp=84921341357&partnerID=8YFLogxK
U2 - 10.2217/fon.14.165
DO - 10.2217/fon.14.165
M3 - Review article
C2 - 25066711
AN - SCOPUS:84921341357
SN - 1479-6694
VL - 11
SP - 233
EP - 249
JO - Future Oncology
JF - Future Oncology
IS - 2
ER -