Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile

Luciane Tsukamoto Kagohara, Juliana L. Schussel, Tejaswini Subbannayya, Nandini Sahasrabuddhe, Cynthia Lebron, Mariana Brait, Leonel Maldonado, Blanca L. Valle, Francesca Pirini, Martha Jahuira, Jaime Lopez, Pablo Letelier, Priscilla Brebi-Mieville, Carmen Ili, Akhilesh Pandey, Aditi Chatterjee, David Sidransky, Rafael Guerrero-Preston

Research output: Contribution to journalReview article

11 Scopus citations

Abstract

Aim: The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. Material & methods: DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR. Results: Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01) and ESR1 (p = 0.05) were significantly different in GBC when compared with cholecystitis. Receiver curve operator analysis revealed promoter methylation of APC, CDKN2A, ESR1, PGP9.5 and SSBP2, together with the Global DNA Methylation Index, had 71% sensitivity, 95% specificity, a 0.97 area under the curve and a positive predictive value of 90%. Conclusion: Global and gene-specific DNA methylation may be useful biomarkers for GBC clinical assessment.

Original languageEnglish (US)
Pages (from-to)233-249
Number of pages17
JournalFuture Oncology
Volume11
Issue number2
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Keywords

  • gallbladder cancer
  • global and gene-specific DNA methylation
  • molecular biomarkers panel
  • tumor suppressor genes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kagohara, L. T., Schussel, J. L., Subbannayya, T., Sahasrabuddhe, N., Lebron, C., Brait, M., Maldonado, L., Valle, B. L., Pirini, F., Jahuira, M., Lopez, J., Letelier, P., Brebi-Mieville, P., Ili, C., Pandey, A., Chatterjee, A., Sidransky, D., & Guerrero-Preston, R. (2015). Global and gene-specific DNA methylation pattern discriminates cholecystitis from gallbladder cancer patients in Chile. Future Oncology, 11(2), 233-249. https://doi.org/10.2217/fon.14.165