GFP-centrin as a marker for centriole dynamics in the human breast cancer cell line MCF-7.

A. B. D'Assoro, F. Stivala, S. Barrett, G. Ferrigno, J. L. Salisbury

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Centrosome duplication plays an important role in genomic stability through bipolar spindle formation and equal chromosome segregation during mitosis. Defects in centrosome duplication and centrosome amplification correlate with aggressive tumors and aneuploidy. Cyclin-dependent cell cycle regulators play a key role in signaling centrosome duplication and the tumor suppressor genes p53, BRCA1 and BRCA2 are suspected to function at mitotic checkpoints that monitor centrosome duplication. The relationship between loss of hormone dependence in breast cancer, and signaling of centrosome duplication in tumor progression is not known. We have developed a MCF-7 cell line expressing GFP-centrin that allows direct visualization of centriole duplication during the cell cycle in living cells. GFP-centrin is expressed and selectively incorporated into the structure of both centrioles making them clearly visible in living cells. Our studies demonstrate three important aspects of recombinant GFP-centrin incorporation into centrioles. 1) GFP-centrin transfected cells grow normally in culture and show no adverse effect associated with GFP-centrin expression; 2) newly duplicated centrioles incorporate centrin during their genesis; and 3) GFP-centrin incorporation into centrioles does not grossly affect cell cycle progression, or centrosome function.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalItalian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia
Volume106
Issue number2 Suppl 1
StatePublished - 2001

ASJC Scopus subject areas

  • Anatomy
  • Embryology

Fingerprint Dive into the research topics of 'GFP-centrin as a marker for centriole dynamics in the human breast cancer cell line MCF-7.'. Together they form a unique fingerprint.

  • Cite this