GFP-centrin as a marker for centriole dynamics in living cells

Richard A. White; Zhang Pan; Jeffrey L. Salisbury

doi:10.1002/(SICI)1097-0029(20000601)49:5<451::AID-JEMT7>3.0.CO;2-9

GFP-centrin as a marker for centriole dynamics in living cells

Richard A. White, Zhang Pan, Jeffrey L. Salisbury

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

A long-standing puzzle in cell biology is the question of how cells generate one and only one new centrosome in each cell cycle and what is the role of the centriole pair in this process. In this study, the introduction of GFP-centrin into cultured cells allows direct visualization of centriole behavior in living cells and in real time. Using this method, centriole dynamics can be observed throughout the cell cycle and following a variety of experimental treatments. Our studies demonstrate that the biogenesis of new centrioles from individual members of a preexisting centriole pair is asynchronous: the older centriole initiates assembly of a new daughter centriole before the younger centriole initiates assembly of its daughter. (C) 2000 Wiley-Liss, Inc.

Original language	English (US)
Pages (from-to)	451-457
Number of pages	7
Journal	Microscopy Research and Technique
Volume	49
Issue number	5
DOIs	https://doi.org/10.1002/(SICI)1097-0029(20000601)49:5<451::AID-JEMT7>3.0.CO;2-9
State	Published - Jun 1 2000

Keywords

Centrosome
GFP-centrin
Mitosis

ASJC Scopus subject areas

Anatomy
Histology
Instrumentation
Medical Laboratory Technology

Access to Document

10.1002/(SICI)1097-0029(20000601)49:5<451::AID-JEMT7>3.0.CO;2-9

Cite this

@article{fc82cc2ec50841259d8e4ce56ab71014,

title = "GFP-centrin as a marker for centriole dynamics in living cells",

abstract = "A long-standing puzzle in cell biology is the question of how cells generate one and only one new centrosome in each cell cycle and what is the role of the centriole pair in this process. In this study, the introduction of GFP-centrin into cultured cells allows direct visualization of centriole behavior in living cells and in real time. Using this method, centriole dynamics can be observed throughout the cell cycle and following a variety of experimental treatments. Our studies demonstrate that the biogenesis of new centrioles from individual members of a preexisting centriole pair is asynchronous: the older centriole initiates assembly of a new daughter centriole before the younger centriole initiates assembly of its daughter. (C) 2000 Wiley-Liss, Inc.",

keywords = "Centrosome, GFP-centrin, Mitosis",

author = "White, {Richard A.} and Zhang Pan and Salisbury, {Jeffrey L.}",

year = "2000",

month = jun,

day = "1",

doi = "10.1002/(SICI)1097-0029(20000601)49:5<451::AID-JEMT7>3.0.CO;2-9",

language = "English (US)",

volume = "49",

pages = "451--457",

journal = "Microscopy Research and Technique",

issn = "1059-910X",

publisher = "Wiley-Liss Inc.",

number = "5",

}

TY - JOUR

T1 - GFP-centrin as a marker for centriole dynamics in living cells

AU - White, Richard A.

AU - Pan, Zhang

AU - Salisbury, Jeffrey L.

PY - 2000/6/1

Y1 - 2000/6/1

N2 - A long-standing puzzle in cell biology is the question of how cells generate one and only one new centrosome in each cell cycle and what is the role of the centriole pair in this process. In this study, the introduction of GFP-centrin into cultured cells allows direct visualization of centriole behavior in living cells and in real time. Using this method, centriole dynamics can be observed throughout the cell cycle and following a variety of experimental treatments. Our studies demonstrate that the biogenesis of new centrioles from individual members of a preexisting centriole pair is asynchronous: the older centriole initiates assembly of a new daughter centriole before the younger centriole initiates assembly of its daughter. (C) 2000 Wiley-Liss, Inc.

AB - A long-standing puzzle in cell biology is the question of how cells generate one and only one new centrosome in each cell cycle and what is the role of the centriole pair in this process. In this study, the introduction of GFP-centrin into cultured cells allows direct visualization of centriole behavior in living cells and in real time. Using this method, centriole dynamics can be observed throughout the cell cycle and following a variety of experimental treatments. Our studies demonstrate that the biogenesis of new centrioles from individual members of a preexisting centriole pair is asynchronous: the older centriole initiates assembly of a new daughter centriole before the younger centriole initiates assembly of its daughter. (C) 2000 Wiley-Liss, Inc.

KW - Centrosome

KW - GFP-centrin

KW - Mitosis

UR - http://www.scopus.com/inward/record.url?scp=0034214267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034214267&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0029(20000601)49:5<451::AID-JEMT7>3.0.CO;2-9

DO - 10.1002/(SICI)1097-0029(20000601)49:5<451::AID-JEMT7>3.0.CO;2-9

M3 - Article

C2 - 10842372

AN - SCOPUS:0034214267

SN - 1059-910X

VL - 49

SP - 451

EP - 457

JO - Microscopy Research and Technique

JF - Microscopy Research and Technique

IS - 5

ER -

GFP-centrin as a marker for centriole dynamics in living cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this