GFP-centrin as a marker for centriole dynamics in living cells

Richard A. White, Zhang Pan, Jeffrey L. Salisbury

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

A long-standing puzzle in cell biology is the question of how cells generate one and only one new centrosome in each cell cycle and what is the role of the centriole pair in this process. In this study, the introduction of GFP-centrin into cultured cells allows direct visualization of centriole behavior in living cells and in real time. Using this method, centriole dynamics can be observed throughout the cell cycle and following a variety of experimental treatments. Our studies demonstrate that the biogenesis of new centrioles from individual members of a preexisting centriole pair is asynchronous: the older centriole initiates assembly of a new daughter centriole before the younger centriole initiates assembly of its daughter. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)451-457
Number of pages7
JournalMicroscopy Research and Technique
Volume49
Issue number5
DOIs
StatePublished - Jun 1 2000

Keywords

  • Centrosome
  • GFP-centrin
  • Mitosis

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

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