TY - JOUR
T1 - Germline mutations in the BRIP1, BARD1, PALB2, and NBN genes in women with ovarian cancer
AU - Ramus, Susan J.
AU - Song, Honglin
AU - Dicks, Ed
AU - Tyrer, Jonathan P.
AU - Rosenthal, Adam N.
AU - Intermaggio, Maria P.
AU - Fraser, Lindsay
AU - Gentry-Maharaj, Aleksandra
AU - Hayward, Jane
AU - Philpott, Susan
AU - Anderson, Christopher
AU - Edlund, Christopher K.
AU - Conti, David
AU - Harrington, Patricia
AU - Barrowdale, Daniel
AU - Bowtell, David D.
AU - Alsop, Kathryn
AU - Mitchell, Gillian
AU - Cicek, Mine S.
AU - Cunningham, Julie M.
AU - Fridley, Brooke L.
AU - Alsop, Jennifer
AU - Jimenez-Linan, Mercedes
AU - Poblete, Samantha
AU - Lele, Shashi
AU - Sucheston-Campbell, Lara
AU - Moysich, Kirsten B.
AU - Sieh, Weiva
AU - McGuire, Valerie
AU - Lester, Jenny
AU - Bogdanova, Natalia
AU - Dürst, Matthias
AU - Hillemanns, Peter
AU - Odunsi, Kunle
AU - Whittemore, Alice S.
AU - Karlan, Beth Y.
AU - Dörk, Thilo
AU - Goode, Ellen L.
AU - Menon, Usha
AU - Jacobs, Ian J.
AU - Antoniou, Antonis C.
AU - Pharoah, Paul D.P.
AU - Gayther, Simon A.
N1 - Funding Information:
This work was funded by the Cancer Councils of New South Wales, Victoria, Queensland, South Australia, and Tasmania, the Cancer Foundation of Western Australia, Cancer Research UK (C1005/A7749, C315/A2621, C490/A10119, C490/A10124, C490/A16561, C1005/A12677, C1005/A6383), the Eve Appeal (The Oak Foundation), the Fred C. and Katherine B. Andersen Foundation, the National Institutes for Health (P30 CA016056, P30-CA15083, P50CA136393, P50CA159981, R01CA122443, R01CA178535, R01CA61107, R01CA152990, and R01CA086381), the National Health & Medical Research Council of Australia (NHMRC; ID400413, ID400281), Cancer Australia (509303), Roswell Park Cancer Institute Alliance Foundation, the UK Department of Health, the UK National Institute for Health Research Biomedical Research Centres at the University of Cambridge and University College London Hospitals Biomedical Research Centre, and the US Army Medical Research and Materiel Command (DAMD17-01-1-0729; W81XWH-08-1-0684 and W81XWH-08-1-0685). The project described was also supported in part by award number P30CA014089 from the National Cancer Institute.
PY - 2015
Y1 - 2015
N2 - Background: Epithelial ovarian cancer (EOC) is the most lethal gynecological malignancy, responsible for 13 000 deaths per year in the United States. Risk prediction based on identifying germline mutations in ovarian cancer susceptibility genes could have a clinically significant impact on reducing disease mortality. Methods: Next generation sequencing was used to identify germline mutations in the coding regions of four candidate susceptibility genes-BRIP1, BARD1, PALB2 and NBN-in 3236 invasive EOC case patients and 3431 control patients of European origin, and in 2000 unaffected high-risk women from a clinical screening trial of ovarian cancer (UKFOCSS). For each gene, we estimated the prevalence and EOC risks and evaluated associations between germline variant status and clinical and epidemiological risk factor information. All statistical tests were two-sided. Results: We found an increased frequency of deleterious mutations in BRIP1 in case patients (0.9%) and in the UKFOCSS participants (0.6%) compared with control patients (0.09%) (P = 1 x 10-4 and 8 x 10-4, respectively), but no differences for BARD1 (P = .39), NBN1 (P = .61), or PALB2 (P = .08). There was also a difference in the frequency of rare missense variants in BRIP1 between case patients and control patients (P = 5.5 x 10-4). The relative risks associated with BRIP1 mutations were 11.22 for invasive EOC (95% confidence interval [CI] = 3.22 to 34.10, P = 1 x 10-4) and 14.09 for high-grade serous disease (95% CI = 4.04 to 45.02, P = 2 x 10-5). Segregation analysis in families estimated the average relative risks in BRIP1 mutation carriers compared with the general population to be 3.41 (95% CI = 2.12 to 5.54, P = 7 × 10-7). Conclusions: Deleterious germline mutations in BRIP1 are associated with a moderate increase in EOC risk. These data have clinical implications for risk prediction and prevention approaches for ovarian cancer and emphasize the critical need for risk estimates based on very large sample sizes before genes of moderate penetrance have clinical utility in cancer prevention.
AB - Background: Epithelial ovarian cancer (EOC) is the most lethal gynecological malignancy, responsible for 13 000 deaths per year in the United States. Risk prediction based on identifying germline mutations in ovarian cancer susceptibility genes could have a clinically significant impact on reducing disease mortality. Methods: Next generation sequencing was used to identify germline mutations in the coding regions of four candidate susceptibility genes-BRIP1, BARD1, PALB2 and NBN-in 3236 invasive EOC case patients and 3431 control patients of European origin, and in 2000 unaffected high-risk women from a clinical screening trial of ovarian cancer (UKFOCSS). For each gene, we estimated the prevalence and EOC risks and evaluated associations between germline variant status and clinical and epidemiological risk factor information. All statistical tests were two-sided. Results: We found an increased frequency of deleterious mutations in BRIP1 in case patients (0.9%) and in the UKFOCSS participants (0.6%) compared with control patients (0.09%) (P = 1 x 10-4 and 8 x 10-4, respectively), but no differences for BARD1 (P = .39), NBN1 (P = .61), or PALB2 (P = .08). There was also a difference in the frequency of rare missense variants in BRIP1 between case patients and control patients (P = 5.5 x 10-4). The relative risks associated with BRIP1 mutations were 11.22 for invasive EOC (95% confidence interval [CI] = 3.22 to 34.10, P = 1 x 10-4) and 14.09 for high-grade serous disease (95% CI = 4.04 to 45.02, P = 2 x 10-5). Segregation analysis in families estimated the average relative risks in BRIP1 mutation carriers compared with the general population to be 3.41 (95% CI = 2.12 to 5.54, P = 7 × 10-7). Conclusions: Deleterious germline mutations in BRIP1 are associated with a moderate increase in EOC risk. These data have clinical implications for risk prediction and prevention approaches for ovarian cancer and emphasize the critical need for risk estimates based on very large sample sizes before genes of moderate penetrance have clinical utility in cancer prevention.
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U2 - 10.1093/jnci/djv214
DO - 10.1093/jnci/djv214
M3 - Article
C2 - 26315354
AN - SCOPUS:84952641661
VL - 107
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 11
M1 - djv214
ER -