Germline BRCA1 mutation reprograms breast epithelial cell metabolism towards mitochondrial-dependent biosynthesis: Evidence for metforminbased "starvation" strategies in BRCA1 carriers

Elisabet Cuyàs, Salvador Fernández-Arroyo, Tomás Alarcón, Ruth Lupu, Jorge Joven, Javier A. Menendez

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We hypothesized that women inheriting one germline mutation of the BRCA1 gene ("one-hit") undergo cell-type-specific metabolic reprogramming that supports the high biosynthetic requirements of breast epithelial cells to progress to a fully malignant phenotype. Targeted metabolomic analysis was performed in isogenic pairs of nontumorigenic human breast epithelial cells in which the knock-in of 185delAG mutation in a single BRCA1 allele leads to genomic instability. Mutant BRCA1 one-hit epithelial cells displayed constitutively enhanced activation of biosynthetic nodes within mitochondria. This metabolic rewiring involved the increased incorporation of glutamine- and glucosedependent carbon into tricarboxylic acid (TCA) cycle metabolite pools to ultimately generate elevated levels of acetyl-CoA and malonyl-CoA, the major building blocks for lipid biosynthesis. The significant increase of branched-chain amino acids (BCAAs) including the anabolic trigger leucine, which can not only promote protein translation via mTOR but also feed into the TCA cycle via succinyl-CoA, further underscored the anabolic reprogramming of BRCA1 haploinsufficient cells. The anti-diabetic biguanide metformin "reversed" the metabolomic signature and anabolic phenotype of BRCA1 one-hit cells by shutting down mitochondria-driven generation of precursors for lipogenic pathways and reducing the BCAA pool for protein synthesis and TCA fueling. Metformin-induced restriction of mitochondrial biosynthetic capacity was sufficient to impair the tumorinitiating capacity of BRCA1 one-hit cells in mammosphere assays. Metabolic rewiring of the breast epithelium towards increased anabolism might constitute an unanticipated and inherited form of metabolic reprogramming linked to increased risk of oncogenesis in women bearing pathogenic germline BRCA1 mutations. The ability of metformin to constrain the production of mitochondrial-dependent biosynthetic intermediates might open a new avenue for "starvation" chemopreventive strategies in BRCA1 carriers.

Original languageEnglish (US)
Pages (from-to)52974-52992
Number of pages19
JournalOncotarget
Volume7
Issue number33
DOIs
StatePublished - Aug 1 2016

Fingerprint

Germ-Line Mutation
Starvation
Metformin
Breast
Epithelial Cells
Branched Chain Amino Acids
Metabolomics
Citric Acid Cycle
Mitochondria
Tricarboxylic Acids
Malonyl Coenzyme A
BRCA1 Gene
Biguanides
Phenotype
Acetyl Coenzyme A
Genomic Instability
Protein Biosynthesis
Glutamine
Leucine
Carcinogenesis

Keywords

  • Bioenergetics
  • BRCA1
  • Breast cancer susceptibility
  • Glutamine
  • Hereditary breast cancer

ASJC Scopus subject areas

  • Oncology

Cite this

Germline BRCA1 mutation reprograms breast epithelial cell metabolism towards mitochondrial-dependent biosynthesis : Evidence for metforminbased "starvation" strategies in BRCA1 carriers. / Cuyàs, Elisabet; Fernández-Arroyo, Salvador; Alarcón, Tomás; Lupu, Ruth; Joven, Jorge; Menendez, Javier A.

In: Oncotarget, Vol. 7, No. 33, 01.08.2016, p. 52974-52992.

Research output: Contribution to journalArticle

Cuyàs, Elisabet ; Fernández-Arroyo, Salvador ; Alarcón, Tomás ; Lupu, Ruth ; Joven, Jorge ; Menendez, Javier A. / Germline BRCA1 mutation reprograms breast epithelial cell metabolism towards mitochondrial-dependent biosynthesis : Evidence for metforminbased "starvation" strategies in BRCA1 carriers. In: Oncotarget. 2016 ; Vol. 7, No. 33. pp. 52974-52992.
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