Genotypic analysis of multiple loci in somatic cells by whole genome amplification

Michael T. Barrett, Brian J. Reid, Geoffrey Joslyn

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.

Original languageEnglish (US)
Pages (from-to)3488-3492
Number of pages5
JournalNucleic acids research
Volume23
Issue number17
DOIs
StatePublished - Sep 11 1995

ASJC Scopus subject areas

  • Genetics

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