Abstract
To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3488-3492 |
| Number of pages | 5 |
| Journal | Nucleic acids research |
| Volume | 23 |
| Issue number | 17 |
| DOIs | |
| State | Published - Sep 11 1995 |
ASJC Scopus subject areas
- Genetics
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Cite this
Barrett, M. T., Reid, B. J., & Joslyn, G. (1995). Genotypic analysis of multiple loci in somatic cells by whole genome amplification. Nucleic acids research, 23(17), 3488-3492. https://doi.org/10.1093/nar/23.17.3488