Genotype-dependent serologic reactivities in patients infected with hepatitis C virus in the United States

N. N. Zein, Jorge Rakela, D. H. Persing

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective: To evaluate the serologic reactivities in patients infected with different hepatitis C virus (HCV) genotypes to four HCV proteins that are components of the second-generation recombinant immunoblot assay. Material and Methods: Serum samples from 36 patients with chronic HCV infection were obtained. RNA was extracted by using chaotropic lysis and isopropanol precipitation. Reverse-transcriptase polymerase chain reaction of the NS-5 region was performed, followed by automated single-pass dideoxy sequencing of desalted amplification products. Classification of isolated HCV subtypes was based on Simmonds' system. All samples were tested for antibodies to proteins 5-1-1, C100-3, C33c, and C22-3 with the second- generation recombinant immunoblot assay. Results: Reactivity to protein 5-1- 1 was significantly lower for patients with genotypes 2b and 3a than for those infected with HCV types 1a or 1b (P<0.05). Antibody reactivity to the C100-3 protein was also reduced in patients infected with HCV types 2b and 3a. Conclusion: These data indicate that the genotype-dependent differences in serologic reactivities are substantial among patients with chronic HCV infection.

Original languageEnglish (US)
Pages (from-to)449-452
Number of pages4
JournalMayo Clinic Proceedings
Volume70
Issue number5
StatePublished - 1995

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Hepacivirus
Genotype
Chronic Hepatitis C
Virus Diseases
Proteins
Viral Structures
2-Propanol
Antibodies
Reverse Transcriptase Polymerase Chain Reaction
RNA
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Genotype-dependent serologic reactivities in patients infected with hepatitis C virus in the United States. / Zein, N. N.; Rakela, Jorge; Persing, D. H.

In: Mayo Clinic Proceedings, Vol. 70, No. 5, 1995, p. 449-452.

Research output: Contribution to journalArticle

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