Genomics of human intracranial aneurysm wall

Changbin Shi, Issam A. Awad, Nadereh Jafari, Simon Lin, Pan Du, Ziad A. Hage, Robert Shenkar, Christopher C. Getch, Markus Bredel, H. Hunt Batjer, Bernard Bendok

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Background and Purpose - The pathogenesis of intracranial aneurysms (IAs) remains elusive. Most studies have focused on individual genes, or a few interrelated genes or products, at a time in human IA. However, a broad view of pathologic mechanisms has not been investigated by identifying pathogenic genes and their interaction in networks. Our study aimed to analyze global gene expression patterns in the IA wall. Methods - To our knowledge, our group was the first to perform Illumina microarray analysis on human IA via comparison of aneurysm wall and superficial temporal artery tissues from 6 consecutive patients. We adopted stringent statistical criteria to the individual genes; genes with a false discovery rate <0.01 and >2-fold change were selected as differentially expressed. To identify the overrepresented biologic pathways with the differentially expressed genes, we performed hypergeometric testing of the genes selected by relaxed criteria of P<0.01 and fold change >1.5. Results - There are 326 distinct differentially expressed genes between IA and superficial temporal artery tissues (>2-fold change) with a false discovery rate <0.01. Analysis of the Kyoto Encyclopedia of Genes and Genomes pathways revealed the most impacted functional pathways: focal adhesion, extracellular matrix receptor interaction, and cell communication. Analysis of the Gene Ontology also supported the involvement of another 2 potentially important pathways: inflammatory response and apoptosis. Conclusions - The differentially expressed genes in the aneurysm wall may shed light on aneurysm pathobiology and provide novel targets for therapeutic intervention. These data will help generate hypotheses for future studies.

Original languageEnglish (US)
Pages (from-to)1252-1261
Number of pages10
JournalStroke
Volume40
Issue number4
DOIs
StatePublished - Apr 1 2009
Externally publishedYes

Fingerprint

Intracranial Aneurysm
Genomics
Genes
Aneurysm
Temporal Arteries
Encyclopedias
Gene Ontology
Focal Adhesions
Gene Expression Profiling
Microarray Analysis
Cell Communication
Genome

Keywords

  • Acute stroke
  • Cerebral aneurysms
  • Gene expression
  • Intracranial aneurysms
  • Microarray

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing
  • Medicine(all)

Cite this

Shi, C., Awad, I. A., Jafari, N., Lin, S., Du, P., Hage, Z. A., ... Bendok, B. (2009). Genomics of human intracranial aneurysm wall. Stroke, 40(4), 1252-1261. https://doi.org/10.1161/STROKEAHA.108.532036

Genomics of human intracranial aneurysm wall. / Shi, Changbin; Awad, Issam A.; Jafari, Nadereh; Lin, Simon; Du, Pan; Hage, Ziad A.; Shenkar, Robert; Getch, Christopher C.; Bredel, Markus; Batjer, H. Hunt; Bendok, Bernard.

In: Stroke, Vol. 40, No. 4, 01.04.2009, p. 1252-1261.

Research output: Contribution to journalArticle

Shi, C, Awad, IA, Jafari, N, Lin, S, Du, P, Hage, ZA, Shenkar, R, Getch, CC, Bredel, M, Batjer, HH & Bendok, B 2009, 'Genomics of human intracranial aneurysm wall', Stroke, vol. 40, no. 4, pp. 1252-1261. https://doi.org/10.1161/STROKEAHA.108.532036
Shi C, Awad IA, Jafari N, Lin S, Du P, Hage ZA et al. Genomics of human intracranial aneurysm wall. Stroke. 2009 Apr 1;40(4):1252-1261. https://doi.org/10.1161/STROKEAHA.108.532036
Shi, Changbin ; Awad, Issam A. ; Jafari, Nadereh ; Lin, Simon ; Du, Pan ; Hage, Ziad A. ; Shenkar, Robert ; Getch, Christopher C. ; Bredel, Markus ; Batjer, H. Hunt ; Bendok, Bernard. / Genomics of human intracranial aneurysm wall. In: Stroke. 2009 ; Vol. 40, No. 4. pp. 1252-1261.
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