Genomic organization and tissue expression of mouse proteasome gene Lmp-2

P. Zhou, E. Zanelli, M. Smart, C. David

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The mouse Lmp-2 gene is located within the major histocompatibility complex (MHC) class II region and encodes a subunit of the 20S cytosolic proteasome. Previous studies indicated that the 20S proteasome is a catalytic core of the 26S proteolytic complex that possesses a latent multicatalytic proteinase activity and catalyzes an ATP-dependent, selective breakdown of proteins ligated to ubiquitin. This complex has recently been postulated to be involved in the processing of endogenous antigenic peptides for the MHC class I pathway. Here, we report the genomic organization and tissue expression of the mouse Lmp-2 gene. We have cloned and sequenced the entire mouse Lmp-2 gene, including 5'- and 3'-flanking regions. The gene consists of six exons, and its genomic organization is very similar to that of the recently described human LMP2 gene. Putative promoter and enhancer elements were identified in the 5'-flanking region by sequence comparison with known consensus sequences. The Lmp-2 gene is expressed in most tissues of unstimulated mice, except for brain tissue. The comparison of the 5'-flanking region of human and mouse sequences is discussed.

Original languageEnglish (US)
Pages (from-to)664-668
Number of pages5
JournalGenomics
Volume16
Issue number3
DOIs
StatePublished - 1993

Fingerprint

Proteasome Endopeptidase Complex
5' Flanking Region
Genes
Major Histocompatibility Complex
3' Flanking Region
Consensus Sequence
Ubiquitin
Exons
Catalytic Domain
Adenosine Triphosphate
Peptides
Brain
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic organization and tissue expression of mouse proteasome gene Lmp-2. / Zhou, P.; Zanelli, E.; Smart, M.; David, C.

In: Genomics, Vol. 16, No. 3, 1993, p. 664-668.

Research output: Contribution to journalArticle

Zhou, P, Zanelli, E, Smart, M & David, C 1993, 'Genomic organization and tissue expression of mouse proteasome gene Lmp-2', Genomics, vol. 16, no. 3, pp. 664-668. https://doi.org/10.1006/geno.1993.1245
Zhou, P. ; Zanelli, E. ; Smart, M. ; David, C. / Genomic organization and tissue expression of mouse proteasome gene Lmp-2. In: Genomics. 1993 ; Vol. 16, No. 3. pp. 664-668.
@article{f3041eff806642be9b73e640dd265af6,
title = "Genomic organization and tissue expression of mouse proteasome gene Lmp-2",
abstract = "The mouse Lmp-2 gene is located within the major histocompatibility complex (MHC) class II region and encodes a subunit of the 20S cytosolic proteasome. Previous studies indicated that the 20S proteasome is a catalytic core of the 26S proteolytic complex that possesses a latent multicatalytic proteinase activity and catalyzes an ATP-dependent, selective breakdown of proteins ligated to ubiquitin. This complex has recently been postulated to be involved in the processing of endogenous antigenic peptides for the MHC class I pathway. Here, we report the genomic organization and tissue expression of the mouse Lmp-2 gene. We have cloned and sequenced the entire mouse Lmp-2 gene, including 5'- and 3'-flanking regions. The gene consists of six exons, and its genomic organization is very similar to that of the recently described human LMP2 gene. Putative promoter and enhancer elements were identified in the 5'-flanking region by sequence comparison with known consensus sequences. The Lmp-2 gene is expressed in most tissues of unstimulated mice, except for brain tissue. The comparison of the 5'-flanking region of human and mouse sequences is discussed.",
author = "P. Zhou and E. Zanelli and M. Smart and C. David",
year = "1993",
doi = "10.1006/geno.1993.1245",
language = "English (US)",
volume = "16",
pages = "664--668",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Genomic organization and tissue expression of mouse proteasome gene Lmp-2

AU - Zhou, P.

AU - Zanelli, E.

AU - Smart, M.

AU - David, C.

PY - 1993

Y1 - 1993

N2 - The mouse Lmp-2 gene is located within the major histocompatibility complex (MHC) class II region and encodes a subunit of the 20S cytosolic proteasome. Previous studies indicated that the 20S proteasome is a catalytic core of the 26S proteolytic complex that possesses a latent multicatalytic proteinase activity and catalyzes an ATP-dependent, selective breakdown of proteins ligated to ubiquitin. This complex has recently been postulated to be involved in the processing of endogenous antigenic peptides for the MHC class I pathway. Here, we report the genomic organization and tissue expression of the mouse Lmp-2 gene. We have cloned and sequenced the entire mouse Lmp-2 gene, including 5'- and 3'-flanking regions. The gene consists of six exons, and its genomic organization is very similar to that of the recently described human LMP2 gene. Putative promoter and enhancer elements were identified in the 5'-flanking region by sequence comparison with known consensus sequences. The Lmp-2 gene is expressed in most tissues of unstimulated mice, except for brain tissue. The comparison of the 5'-flanking region of human and mouse sequences is discussed.

AB - The mouse Lmp-2 gene is located within the major histocompatibility complex (MHC) class II region and encodes a subunit of the 20S cytosolic proteasome. Previous studies indicated that the 20S proteasome is a catalytic core of the 26S proteolytic complex that possesses a latent multicatalytic proteinase activity and catalyzes an ATP-dependent, selective breakdown of proteins ligated to ubiquitin. This complex has recently been postulated to be involved in the processing of endogenous antigenic peptides for the MHC class I pathway. Here, we report the genomic organization and tissue expression of the mouse Lmp-2 gene. We have cloned and sequenced the entire mouse Lmp-2 gene, including 5'- and 3'-flanking regions. The gene consists of six exons, and its genomic organization is very similar to that of the recently described human LMP2 gene. Putative promoter and enhancer elements were identified in the 5'-flanking region by sequence comparison with known consensus sequences. The Lmp-2 gene is expressed in most tissues of unstimulated mice, except for brain tissue. The comparison of the 5'-flanking region of human and mouse sequences is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0027308710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027308710&partnerID=8YFLogxK

U2 - 10.1006/geno.1993.1245

DO - 10.1006/geno.1993.1245

M3 - Article

C2 - 8325639

AN - SCOPUS:0027308710

VL - 16

SP - 664

EP - 668

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -