Genomic Organization and Expression Analysis of B7-H4, an Immune Inhibitory Molecule of the B7 Family

In Hak Choi, Gefeng Zhu, Gabriel L. Sica, Scott E. Strome, John C. Cheville, Julie S. Lau, Yuwen Zhu, Dallas B. Flies, Koji Tamada, Lieping Chen

Research output: Contribution to journalArticle

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Abstract

B7-H4 is a recently identified B7 family member that negatively regulates T cell immunity by the inhibition of T cell proliferation, cytokine production, and cell cycle progression. In this study, we report that the genomic DNA of human B7-H4 is mapped on chromosome 1 comprised of six exons and five introns spanning 66 kb, of which exon 6 is used for alternative splicing to generate two different transcripts. Similar B7-H4 structure is also found in mouse genomic DNA in chromosome 3. A human B7-H4 pseudogene is identified in chromosome 20p11.1 with a single exon and two stop codons in the coding region. Immunohistochemistry analysis using B7-H4-specific mAb demonstrates that B7-H4 is not expressed on the majority of normal human tissues. In contrast, up to 85% (22 of 26) of ovarian cancer and 31% (5 of 16) of lung cancer tissues constitutively express B7-H4. Our results indicate a tight regulation of B7-H4 expression in the translational level in normal peripheral tissues and a potential role of B7-H4 in the evasion of tumor immunity.

Original languageEnglish (US)
Pages (from-to)4650-4654
Number of pages5
JournalJournal of Immunology
Volume171
Issue number9
StatePublished - Oct 1 2003

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B7 Antigens
Exons
Immunity
T-Lymphocytes
Pseudogenes
Chromosomes, Human, Pair 3
Terminator Codon
Chromosomes, Human, Pair 1
DNA
Alternative Splicing
Ovarian Neoplasms
Introns
Lung Neoplasms
Cell Cycle
Chromosomes
Immunohistochemistry
Cell Proliferation
Cytokines
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Choi, I. H., Zhu, G., Sica, G. L., Strome, S. E., Cheville, J. C., Lau, J. S., ... Chen, L. (2003). Genomic Organization and Expression Analysis of B7-H4, an Immune Inhibitory Molecule of the B7 Family. Journal of Immunology, 171(9), 4650-4654.

Genomic Organization and Expression Analysis of B7-H4, an Immune Inhibitory Molecule of the B7 Family. / Choi, In Hak; Zhu, Gefeng; Sica, Gabriel L.; Strome, Scott E.; Cheville, John C.; Lau, Julie S.; Zhu, Yuwen; Flies, Dallas B.; Tamada, Koji; Chen, Lieping.

In: Journal of Immunology, Vol. 171, No. 9, 01.10.2003, p. 4650-4654.

Research output: Contribution to journalArticle

Choi, IH, Zhu, G, Sica, GL, Strome, SE, Cheville, JC, Lau, JS, Zhu, Y, Flies, DB, Tamada, K & Chen, L 2003, 'Genomic Organization and Expression Analysis of B7-H4, an Immune Inhibitory Molecule of the B7 Family', Journal of Immunology, vol. 171, no. 9, pp. 4650-4654.
Choi IH, Zhu G, Sica GL, Strome SE, Cheville JC, Lau JS et al. Genomic Organization and Expression Analysis of B7-H4, an Immune Inhibitory Molecule of the B7 Family. Journal of Immunology. 2003 Oct 1;171(9):4650-4654.
Choi, In Hak ; Zhu, Gefeng ; Sica, Gabriel L. ; Strome, Scott E. ; Cheville, John C. ; Lau, Julie S. ; Zhu, Yuwen ; Flies, Dallas B. ; Tamada, Koji ; Chen, Lieping. / Genomic Organization and Expression Analysis of B7-H4, an Immune Inhibitory Molecule of the B7 Family. In: Journal of Immunology. 2003 ; Vol. 171, No. 9. pp. 4650-4654.
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